DOI: 10.1084/jem.190.4.451. Malignant Transformation and Antineoplastic Actions of Nonsteroidal Antiinflammatory Drugs (Nsaids) on Cyclooxygenase-Null Embryo Fibroblasts

Malignant Transformation and Antineoplastic Actions of Nonsteroidal Antiinflammatory Drugs (Nsaids) on Cyclooxygenase-Null Embryo Fibroblasts

10.1084/jem.190.4.451

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

In this study, we use primary embryonic fibroblasts derived from cyclooxygenase-deficient transgenic embryos to further investigate the role of the two cyclooxygenases, cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), in the process of neoplastic transformation. Cells with either, neither, or both of the cyclooxygenases were transformed by Ha-ras and/or SV40. Our results show that when a cyclooxygenase enzyme is present, the transformed cells have marked increases in COX-2 and/or COX-1 expression. Nevertheless, each type of cell, deficient in either or both cyclooxygenases, can be readily transformed at almost equal efficiency. Different nonsteroidal antiinflammatory drugs (NSAIDs) were used to examine their possible antineoplastic effects on the transformed cells, which have various levels of expression of COX-1 or COX-2. Our results show that NSAIDs suppress the colony formation in soft agar in a dosage-dependent manner in the absence of the cyclooxygenase(s). Thymidine incorporation and apoptosis analyses further demonstrate that the NSAIDs are effective in the cyclooxygenase-null cells. Our findings with cyclooxygenase knockout cells confirm recent reports that some of the antiproliferative and antineoplastic effects of NSAIDs are independent of the inhibition of either COX-1 or COX-2. They also show that transformation is independent of the status of cyclooxygenase expression, suggesting that the involvement of the cyclooxygenases in tumorigenesis may occur at later steps.

Bibliography

Zhang, X., Morham, S. G., Langenbach, R., & Young, D. A. (1999). Malignant Transformation and Antineoplastic Actions of Nonsteroidal Antiinflammatory Drugs (Nsaids) on Cyclooxygenase-Null Embryo Fibroblasts. The Journal of Experimental Medicine, 190(4), 451â460.

Authors 4

Xinping Zhang (first)
Scott G. Morham (additional)
Robert Langenbach (additional)
Donald A. Young (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 6:05 a.m.)
Indexed	2 months ago (July 1, 2025, 9:48 a.m.)
Issued	26 years ago (Aug. 16, 1999)
Published	26 years ago (Aug. 16, 1999)
Published Online	26 years ago (Aug. 16, 1999)
Published Print	26 years ago (Aug. 16, 1999)

Funders 0

None

BibTeX

@article{Zhang_1999, title={Malignant Transformation and Antineoplastic Actions of Nonsteroidal Antiinflammatory Drugs (Nsaids) on Cyclooxygenase-Null Embryo Fibroblasts}, volume={190}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.190.4.451}, DOI={10.1084/jem.190.4.451}, number={4}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Zhang, Xinping and Morham, Scott G. and Langenbach, Robert and Young, Donald A.}, year={1999}, month=aug, pages={451–460} }

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