DOI: 10.1084/jem.189.6.895. Modulation of Proteasomal Activity Required for the Generation of a Cytotoxic T Lymphocyte–defined Peptide Derived from the Tumor Antigen MAGE-3

Modulation of Proteasomal Activity Required for the Generation of a Cytotoxic T Lymphocyte–defined Peptide Derived from the Tumor Antigen MAGE-3

10.1084/jem.189.6.895

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

We have analyzed the presentation of human histocompatability leukocyte antigen-A*0201–associated tumor peptide antigen MAGE-3271–279 by melanoma cells. We show that specific cytotoxic T lymphocyte (CTL)-recognizing cells transfected with a minigene encoding the preprocessed fragment MAGE-3271–279 failed to recognize cells expressing the full length MAGE-3 protein. Digestion of synthetic peptides extended at the NH2 or COOH terminus of MAGE-3271–279 with purified human proteasome revealed that the generation of the COOH terminus of the antigenic peptide was impaired. Surprisingly, addition of lactacystin to purified proteasome, though partially inhibitory, resulted in the generation of the antigenic peptide. Furthermore, treatment of melanoma cells expressing the MAGE-3 protein with lactacystin resulted in efficient lysis by MAGE-3271–279–specific CTL. We therefore postulate that the generation of antigenic peptides by the proteasome in cells can be modulated by the selective inhibition of certain of its enzymatic activities.

Bibliography

Valmori, D., Gileadi, U., Servis, C., Dunbar, P. R., Cerottini, J.-C., Romero, P., Cerundolo, V., & LÃ©vy, F. (1999). Modulation of Proteasomal Activity Required for the Generation of a Cytotoxic T Lymphocyteâdefined Peptide Derived from the Tumor Antigen MAGE-3. The Journal of Experimental Medicine, 189(6), 895â906.

Authors 8

Danila Valmori (first)
Uzi Gileadi (additional)
Catherine Servis (additional)
P. Rod Dunbar (additional)
Jean-Charles Cerottini (additional)
Pedro Romero (additional)
Vincenzo Cerundolo (additional)
Frédéric Lévy (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 5:51 a.m.)
Indexed	3 months, 1 week ago (May 23, 2025, 4:26 a.m.)
Issued	26 years, 5 months ago (March 15, 1999)
Published	26 years, 5 months ago (March 15, 1999)
Published Online	26 years, 5 months ago (March 15, 1999)
Published Print	26 years, 5 months ago (March 15, 1999)

Funders 0

None

BibTeX

@article{Valmori_1999, title={Modulation of Proteasomal Activity Required for the Generation of a Cytotoxic T Lymphocyte–defined Peptide Derived from the Tumor Antigen MAGE-3}, volume={189}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.189.6.895}, DOI={10.1084/jem.189.6.895}, number={6}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Valmori, Danila and Gileadi, Uzi and Servis, Catherine and Dunbar, P. Rod and Cerottini, Jean-Charles and Romero, Pedro and Cerundolo, Vincenzo and Lévy, Frédéric}, year={1999}, month=mar, pages={895–906} }

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