Cytotoxicity Is Mandatory for CD8+ T Cell–mediated Contact Hypersensitivity
10.1084/jem.189.5.779
Crossref journal-article
Rockefeller University Press
The Journal of Experimental Medicine (291)
Abstract

Contact hypersensitivity (CHS) is a T cell–mediated skin inflammation induced by epicutaneous exposure to haptens in sensitized individuals. We have previously reported that CHS to dinitrofluorobenzene in mice is mediated by major histocompatibility complex (MHC) class I–restricted CD8+ T cells. In this study, we show that CD8+ T cells mediate the skin inflammation through their cytotoxic activity. The contribution of specific cytotoxic T lymphocytes (CTLs) to the CHS reaction was examined both in vivo and in vitro, using mice deficient in perforin and/or Fas/Fas ligand (FasL) pathways involved in cytotoxicity. Mice double deficient in perforin and FasL were able to develop hapten-specific CD8+ T cells in the lymphoid organs but did not show CHS reaction. However, they did not generate hapten-specific CTLs, demonstrating that the CHS reaction is dependent on cytotoxic activity. In contrast, Fas-deficient lpr mice, FasL-deficient gld mice, and perforin-deficient mice developed a normal CHS reaction and were able to generate hapten-specific CTLs, suggesting that CHS requires either the Fas/FasL or the perforin pathway. This was confirmed by in vitro studies showing that the hapten-specific CTL activity was exclusively mediated by MHC class I–restricted CD8+ T cells which could use either the perforin or the Fas/FasL pathway for their lytic activity. Thus, cytotoxic CD8+ T cells, commonly implicated in the host defence against tumors and viral infections, could also mediate harmful delayed-type hypersensitivity reactions.

Bibliography

Kehren, J., Desvignes, C., Krasteva, M., Ducluzeau, M.-T., Assossou, O., Horand, F., Hahne, M., Kägi, D., Kaiserlian, D., & Nicolas, J.-F. (1999). Cytotoxicity Is Mandatory for CD8+ T Cell–mediated Contact Hypersensitivity. The Journal of Experimental Medicine, 189(5), 779–786.

Authors 10
  1. Jeanne Kehren (first)
  2. Cyril Desvignes (additional)
  3. Maya Krasteva (additional)
  4. Marie-Thérèse Ducluzeau (additional)
  5. Olga Assossou (additional)
  6. Françoise Horand (additional)
  7. Michael Hahne (additional)
  8. David Kägi (additional)
  9. Dominique Kaiserlian (additional)
  10. Jean-François Nicolas (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited 2 years, 1 month ago (July 25, 2023, 5:52 a.m.)
Indexed 1 month ago (July 27, 2025, 3:46 a.m.)
Issued 26 years, 5 months ago (March 1, 1999)
Published 26 years, 5 months ago (March 1, 1999)
Published Online 26 years, 5 months ago (March 1, 1999)
Published Print 26 years, 5 months ago (March 1, 1999)
Funders 0

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@article{Kehren_1999, title={Cytotoxicity Is Mandatory for CD8+ T Cell–mediated Contact Hypersensitivity}, volume={189}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.189.5.779}, DOI={10.1084/jem.189.5.779}, number={5}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Kehren, Jeanne and Desvignes, Cyril and Krasteva, Maya and Ducluzeau, Marie-Thérèse and Assossou, Olga and Horand, Françoise and Hahne, Michael and Kägi, David and Kaiserlian, Dominique and Nicolas, Jean-François}, year={1999}, month=mar, pages={779–786} }