DOI: 10.1084/jem.189.1.169. DNA Vaccination: Transfection and Activation of Dendritic Cells as Key Events for Immunity

DNA Vaccination: Transfection and Activation of Dendritic Cells as Key Events for Immunity

10.1084/jem.189.1.169

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

The mechanisms underlying initiation and maintenance of CD4 T cell responses after DNA vaccination were studied using a construct coding for nonsecreted fifth component of complement (C5) protein, thus restricting the availability of antigen. The only cell types to express C5 were keratinocytes at the site of DNA application and a small number of dendritic cells present in the draining lymph nodes. Antigen expression persisted for up to 12 wk in keratinocytes, but dendritic cells did not express C5 beyond 2 wk after vaccination. Cross-priming of dendritic cells by C5 expressed in keratinocytes did not occur unless keratinocyte death was induced by irradiation in vitro. CD4 T cells were activated in the draining lymph nodes only and subsequently migrated to the spleen, where memory T cells persisted for longer than 40 wk despite the absence of a source of persistent antigen. While DNA vaccination resulted in transfection of a small proportion of dendritic cells only, it led to general activation of all dendritic cells, thus providing optimal conditions for effective T cell activation and maintenance of memory.

Bibliography

Akbari, O., Panjwani, N., Garcia, S., Tascon, R., Lowrie, D., & Stockinger, B. (1999). DNA Vaccination: âTransfection and Activation of Dendritic Cells as Key Events for Immunity. The Journal of Experimental Medicine, 189(1), 169â178.

Authors 6

Omid Akbari (first)
Naveed Panjwani (additional)
Sylvie Garcia (additional)
Ricardo Tascon (additional)
Doug Lowrie (additional)
Brigitta Stockinger (additional)

References 36 Referenced 281

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:49 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 5:39 a.m.)
Indexed	4 days, 7 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	26 years, 7 months ago (Jan. 4, 1999)
Published	26 years, 7 months ago (Jan. 4, 1999)
Published Online	26 years, 7 months ago (Jan. 4, 1999)
Published Print	26 years, 7 months ago (Jan. 4, 1999)

Funders 0

None

BibTeX

@article{Akbari_1999, title={DNA Vaccination: Transfection and Activation of Dendritic Cells as Key Events for Immunity}, volume={189}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.189.1.169}, DOI={10.1084/jem.189.1.169}, number={1}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Akbari, Omid and Panjwani, Naveed and Garcia, Sylvie and Tascon, Ricardo and Lowrie, Doug and Stockinger, Brigitta}, year={1999}, month=jan, pages={169–178} }

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