DOI: 10.1084/jem.188.9.1751. Rapid Secretion of Prestored Interleukin 8 from Weibel-Palade Bodies of Microvascular Endothelial Cells

Rapid Secretion of Prestored Interleukin 8 from Weibel-Palade Bodies of Microvascular Endothelial Cells

10.1084/jem.188.9.1751

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Interleukin (IL)-8, a C-X-C chemokine, activates integrin-mediated adhesion of neutrophils. Presentation of IL-8 on the endothelial cell surface may promote leukocyte extravasation. We found that cultured human microvascular endothelial cells from the intestine (HIMEC) and from nasal polyps (PMEC), but not human umbilical vein endothelial cells (HUVEC), contained IL-8 in intracellular granules that coexpressed von Willebrand factor (vWf ). This observation was corroborated by the immunohistochemical observation of double-positive granules (IL-8+vWf+) in vessels of small and large intestine, nasal mucosa, and skin, whereas umbilical cords revealed no endothelial IL-8. After treatment of HIMEC or PMEC with histamine or thrombin, a dramatic increase in supernatant IL-8 concentration was observed within 3 min, whereas no increase in IL-8 was detected in supernatants of identically treated HUVEC cultures. Histamine or thrombin treatment also caused IL-8–containing granules to rapidly disappear from HIMEC. In HUVEC, IL-8–containing granules were inducible by treatment with recombinant human IL-1β for 24 h; additional histamine treatment doubled IL-8 secretion from HUVEC in the same rapid manner observed for mucosal EC. These data suggested that IL-8 prestored in microvascular endothelial cells may provide a rapid pathway for specific activation of neutrophil adhesion at sites of acute inflammation.

Bibliography

Utgaard, J. O., Jahnsen, F. L., Bakka, A., Brandtzaeg, P., & Haraldsen, G. (1998). Rapid Secretion of Prestored Interleukin 8 from Weibel-Palade Bodies of Microvascular Endothelial Cells. The Journal of Experimental Medicine, 188(9), 1751â1756.

Authors 5

Jon Olav Utgaard (first)
Frode L. Jahnsen (additional)
Arne Bakka (additional)
Per Brandtzaeg (additional)
Guttorm Haraldsen (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:49 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 5:39 a.m.)
Indexed	1 month, 3 weeks ago (July 14, 2025, 11:14 p.m.)
Issued	26 years, 10 months ago (Nov. 2, 1998)
Published	26 years, 10 months ago (Nov. 2, 1998)
Published Online	26 years, 10 months ago (Nov. 2, 1998)
Published Print	26 years, 10 months ago (Nov. 2, 1998)

Funders 0

None

BibTeX

@article{Utgaard_1998, title={Rapid Secretion of Prestored Interleukin 8 from Weibel-Palade Bodies of Microvascular Endothelial Cells}, volume={188}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.188.9.1751}, DOI={10.1084/jem.188.9.1751}, number={9}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Utgaard, Jon Olav and Jahnsen, Frode L. and Bakka, Arne and Brandtzaeg, Per and Haraldsen, Guttorm}, year={1998}, month=nov, pages={1751–1756} }

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  "abstract": "<jats:p>Interleukin (IL)-8, a C-X-C chemokine, activates integrin-mediated adhesion of neutrophils. Presentation of IL-8 on the endothelial cell surface may promote leukocyte extravasation. We found that cultured human microvascular endothelial cells from the intestine (HIMEC) and from nasal polyps (PMEC), but not human umbilical vein endothelial cells (HUVEC), contained IL-8 in intracellular granules that coexpressed von Willebrand factor (vWf\u2009\u2009). This observation was corroborated by the immunohistochemical observation of double-positive granules (IL-8+vWf+) in vessels of small and large intestine, nasal mucosa, and skin, whereas umbilical cords revealed no endothelial IL-8. After treatment of HIMEC or PMEC with histamine or thrombin, a dramatic increase in supernatant IL-8 concentration was observed within 3 min, whereas no increase in IL-8 was detected in supernatants of identically treated HUVEC cultures. Histamine or thrombin treatment also caused IL-8\u2013containing granules to rapidly disappear from HIMEC. In HUVEC, IL-8\u2013containing granules were inducible by treatment with recombinant human IL-1\u03b2 for 24 h; additional histamine treatment doubled IL-8 secretion from HUVEC in the same rapid manner observed for mucosal EC. These data suggested that IL-8 prestored in microvascular endothelial cells may provide a rapid pathway for specific activation of neutrophil adhesion at sites of acute inflammation.</jats:p>",
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