DOI: 10.1084/jem.188.1.17. Cloning of Vascular Adhesion Protein 1 Reveals a Novel Multifunctional Adhesion Molecule

Cloning of Vascular Adhesion Protein 1 Reveals a Novel Multifunctional Adhesion Molecule

10.1084/jem.188.1.17

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Vascular adhesion protein 1 (VAP-1) is a human endothelial sialoglycoprotein whose cell surface expression is induced under inflammatory conditions. It has been shown previously to participate in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin–independent fashion. We report here that the VAP-1 cDNA encodes a type II transmembrane protein of 84.6 kD with a single transmembrane domain located at the NH2-terminal end of the molecule and six potential N-glycosylation sites in the extracellular domain. In vivo, the protein exists predominantly as a homodimer of 170–180 kD. Ax endothelial cells transfected with a VAP-1 cDNA express VAP-1 on their cell surface and bind lymphocytes, and the binding can be partially inhibited with anti–VAP-1 mAbs. VAP-1 has no similarity to any currently known adhesion molecules, but has significant identity to the copper-containing amine oxidase family and has a monoamine oxidase activity. We propose that VAP-1 is a novel type of adhesion molecule with dual function. With the appropriate glycosylation and in the correct inflammatory setting, its expression on the lumenal endothelial cell surface allows it to mediate lymphocyte adhesion and to function as an adhesion receptor involved in lymphocyte recirculation. Its primary function in other locations where it is expressed, such as smooth muscle, may depend on its inherent monoamine oxidase activity.

Bibliography

Smith, D. J., Salmi, M., Bono, P., Hellman, J., Leu, T., & Jalkanen, S. (1998). Cloning of âVascular Adhesion Protein 1 Reveals a Novel Multifunctional Adhesion Molecule. The Journal of Experimental Medicine, 188(1), 17â27.

Authors 6

David J. Smith (first)
Marko Salmi (additional)
Petri Bono (additional)
Jukka Hellman (additional)
Taina Leu (additional)
Sirpa Jalkanen (additional)

References 40 Referenced 243

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:49 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 5:22 a.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 10:29 a.m.)
Issued	27 years, 2 months ago (July 1, 1998)
Published	27 years, 2 months ago (July 1, 1998)
Published Online	27 years, 2 months ago (July 1, 1998)
Published Print	27 years, 2 months ago (July 1, 1998)

Funders 0

None

BibTeX

@article{Smith_1998, title={Cloning of Vascular Adhesion Protein 1 Reveals a Novel Multifunctional Adhesion Molecule}, volume={188}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.188.1.17}, DOI={10.1084/jem.188.1.17}, number={1}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Smith, David J. and Salmi, Marko and Bono, Petri and Hellman, Jukka and Leu, Taina and Jalkanen, Sirpa}, year={1998}, month=jul, pages={17–27} }

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