DOI: 10.1084/jem.187.9.1529. One of the CD3ε Subunits within a T Cell Receptor Complex Lies in Close Proximity to the Cβ FG Loop

One of the CD3ε Subunits within a T Cell Receptor Complex Lies in Close Proximity to the Cβ FG Loop

10.1084/jem.187.9.1529

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

A recent crystal structure of the N15 α/β-T cell receptor (TCR) in complex with an Fab derived from the H57 Cβ-specific monoclonal antibody (mAb) shows the mAb fragment interacting with the elongated FG loop of the Cβ domain. This loop creates one side wall of a cavity within the TCR Ti-α/β constant region module (CαCβ) while the CD and EF loops of the Cα domain form another wall. The cavity size is sufficient to accommodate a single nonglycosylated Ig domain such as the CD3ε ectodomain. By using specific mAbs to mouse TCR-β (H57) and CD3ε (2C11) subunits, we herein provide evidence that only one of the two CD3ε chains within the TCR complex is located in close proximity to the TCR Cβ FG loop, in support of the above notion. Moreover, analysis of T cells isolated from transgenic mice expressing both human and mouse CD3ε genes shows that the heterologous human CD3ε component can replace the mouse CD3ε at this site. The location of one CD3ε subunit within the rigid constant domain module has implications for the mechanism of signal transduction throughout T cell development.

Bibliography

Ghendler, Y., Smolyar, A., Chang, H.-C., & Reinherz, E. L. (1998). One of the CD3Îµ Subunits within a T Cell Receptor Complex Lies in Close Proximity to the CÎ² FG Loop. The Journal of Experimental Medicine, 187(9), 1529â1536.

Authors 4

Yoseph Ghendler (first)
Alex Smolyar (additional)
Hsiu-Ching Chang (additional)
Ellis L. Reinherz (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 4:56 a.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 6:58 p.m.)
Issued	27 years, 3 months ago (May 4, 1998)
Published	27 years, 3 months ago (May 4, 1998)
Published Online	27 years, 3 months ago (May 4, 1998)
Published Print	27 years, 3 months ago (May 4, 1998)

Funders 0

None

BibTeX

@article{Ghendler_1998, title={One of the CD3ε Subunits within a T Cell Receptor Complex Lies in Close Proximity to the Cβ FG Loop}, volume={187}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.187.9.1529}, DOI={10.1084/jem.187.9.1529}, number={9}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Ghendler, Yoseph and Smolyar, Alex and Chang, Hsiu-Ching and Reinherz, Ellis L.}, year={1998}, month=may, pages={1529–1536} }

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