NF-κB RelA-deficient Lymphocytes: Normal Development of  T Cells and B Cells, Impaired Production of IgA and IgG1 and Reduced Proliferative Responses
10.1084/jem.185.5.953
Crossref journal-article
Rockefeller University Press
The Journal of Experimental Medicine (291)
Abstract

To investigate the function of NF-κB RelA (p65), we generated mice deficient in this NF-κB family member by homologous recombination. Mice lacking RelA showed liver degeneration and died around embryonic day 14.5. To elucidate the role of RelA in lymphocyte development and function, we transplanted fetal liver cells of 13.5-day embryos from heterozygote matings into irradiated SCID mice. Within 4 weeks, both T and B cells had developed in the SCID mice receiving relA−/− fetal liver transplants, similar to the relA+/+ and +/− cases. T cells were found to mature to Thy-1+/TCRαβ+/CD3+/CD4+ or CD8+, while B cells had the ability to differentiate to IgM+/B220+ and to secrete immunoglobulins. However, the secretion of IgG1 and IgA was reduced in RelA-deficient B cells. Furthermore, both T and B cells lacking RelA showed marked reduction in proliferative responses to stimulation with Con A, anti-CD3, anti-CD3+anti-CD28, LPS, anti-IgM, and PMA+calcium ionophore. The results indicate that RelA plays a critical role in production of specific Ig isotypes and also in signal transduction pathways for lymphocyte proliferation.

Bibliography

Doi, T. S., Takahashi, T., Taguchi, O., Azuma, T., & Obata, Y. (1997). NF-κB RelA-deficient Lymphocytes: Normal Development of  T Cells and B Cells, Impaired Production of IgA and IgG1 and Reduced Proliferative Responses. The Journal of Experimental Medicine, 185(5), 953–962.

Authors 5
  1. Takahiro S. Doi (first)
  2. Toshitada Takahashi (additional)
  3. Osamu Taguchi (additional)
  4. Takachika Azuma (additional)
  5. Yuichi Obata (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 12:49 p.m.)
Deposited 2 years, 1 month ago (July 25, 2023, 3:51 a.m.)
Indexed 1 month, 4 weeks ago (July 2, 2025, 3:31 a.m.)
Issued 28 years, 5 months ago (March 3, 1997)
Published 28 years, 5 months ago (March 3, 1997)
Published Online 28 years, 5 months ago (March 3, 1997)
Published Print 28 years, 5 months ago (March 3, 1997)
Funders 0

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@article{Doi_1997, title={NF-κB RelA-deficient Lymphocytes: Normal Development of  T Cells and B Cells, Impaired Production of IgA and IgG1 and Reduced Proliferative Responses}, volume={185}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.185.5.953}, DOI={10.1084/jem.185.5.953}, number={5}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Doi, Takahiro S. and Takahashi, Toshitada and Taguchi, Osamu and Azuma, Takachika and Obata, Yuichi}, year={1997}, month=mar, pages={953–962} }