DOI: 10.1084/jem.184.6.2433. Efficient Interaction of HIV-1 with Purified Dendritic Cells via Multiple Chemokine Coreceptors

Efficient Interaction of HIV-1 with Purified Dendritic Cells via Multiple Chemokine Coreceptors

10.1084/jem.184.6.2433

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

HIV-1 actively replicates in dendritic cell (DC)-T cell cocultures, but it has been difficult to demonstrate substantial infection of purified mature DCs. We now find that HIV-1 begins reverse transcription much more efficiently in DCs than T cells, even though T cells have higher levels of CD4 and gp120 binding. DCs isolated from skin or from blood precursors behave similarly. Several M-tropic strains and the T-tropic strain IIIB enter DCs efficiently, as assessed by the progressive formation of the early products of reverse transcription after a 90-min virus pulse at 37°C. However, few late gag-containing sequences are detected, so that active viral replication does not occur. The formation of these early transcripts seems to follow entry of HIV-1, rather than binding of virions that contain viral DNA. Early transcripts are scarce if DCs are exposed to virus on ice for 4 h, or for 90 min at 37°C, conditions which allow virus binding. Also the early transcripts once formed are insensitive to trypsin. The entry of a M-tropic isolates is blocked by the chemokine RANTES, and the entry of IIIB by SDF-1. RANTES interacts with CCR5 and SDF-1 with CXCR4 receptors. Entry of M-tropic but not T-tropic virus is ablated in DCs from individuals who lack a functional CCR5 receptor. DCs express more CCR5 and CXCR4 mRNA than T cells. Therefore, while HIV-1 does not replicate efficiently in mature DCs, viral entry can be active and can be blocked by chemokines that act on known receptors for M- and T-tropic virus.

Bibliography

Granelli-Piperno, A., Moser, B., Pope, M., Chen, D., Wei, Y., Isdell, F., OâDoherty, U., Paxton, W., Koup, R., Mojsov, S., Bhardwaj, N., Clark-Lewis, I., Baggiolini, M., & Steinman, R. M. (1996). Efficient Interaction of HIV-1 with Purified Dendritic Cells via Multiple Chemokine Coreceptors. The Journal of Experimental Medicine, 184(6), 2433â2438.

Authors 14

Angela Granelli-Piperno (first)
Bernhard Moser (additional)
Melissa Pope (additional)
Dongling Chen (additional)
Yang Wei (additional)
Frank Isdell (additional)
Una O'Doherty (additional)
William Paxton (additional)
Richard Koup (additional)
Svetlana Mojsov (additional)
Nina Bhardwaj (additional)
Ian Clark-Lewis (additional)
Marco Baggiolini (additional)
Ralph M. Steinman (additional)

References 33 Referenced 199

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Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years ago (July 28, 2023, 3:51 p.m.)
Indexed	1 month, 4 weeks ago (June 27, 2025, 9:45 a.m.)
Issued	28 years, 8 months ago (Dec. 1, 1996)
Published	28 years, 8 months ago (Dec. 1, 1996)
Published Online	28 years, 8 months ago (Dec. 1, 1996)
Published Print	28 years, 8 months ago (Dec. 1, 1996)

Funders 0

None

BibTeX

@article{Granelli_Piperno_1996, title={Efficient Interaction of HIV-1 with Purified Dendritic Cells via Multiple Chemokine Coreceptors}, volume={184}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.184.6.2433}, DOI={10.1084/jem.184.6.2433}, number={6}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Granelli-Piperno, Angela and Moser, Bernhard and Pope, Melissa and Chen, Dongling and Wei, Yang and Isdell, Frank and O’Doherty, Una and Paxton, William and Koup, Richard and Mojsov, Svetlana and Bhardwaj, Nina and Clark-Lewis, Ian and Baggiolini, Marco and Steinman, Ralph M.}, year={1996}, month=dec, pages={2433–2438} }

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