DOI: 10.1084/jem.184.4.1519. Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients.

Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients.

10.1084/jem.184.4.1519

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

We have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (approximately 90%) were NSE- and CD68-, with only a minor population (approximately 10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)-alpha. These results suggest that NO may mediate pathology in RA through the induction of TNF-alpha production.

Bibliography

McInnes, I. B., Leung, B. P., Field, M., Wei, X. Q., Huang, F. P., Sturrock, R. D., Kinninmonth, A., Weidner, J., Mumford, R., & Liew, F. Y. (1996). Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients. The Journal of Experimental Medicine, 184(4), 1519â1524.

Authors 10

I B McInnes (first)
B P Leung (additional)
M Field (additional)
X Q Wei (additional)
F P Huang (additional)
R D Sturrock (additional)
A Kinninmonth (additional)
J Weidner (additional)
R Mumford (additional)
F Y Liew (additional)

References 0 Referenced 222

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 3:27 a.m.)
Indexed	3 months, 2 weeks ago (May 21, 2025, 4:26 a.m.)
Issued	28 years, 11 months ago (Oct. 1, 1996)
Published	28 years, 11 months ago (Oct. 1, 1996)
Published Online	28 years, 11 months ago (Oct. 1, 1996)
Published Print	28 years, 11 months ago (Oct. 1, 1996)

Funders 0

None

BibTeX

@article{McInnes_1996, title={Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients.}, volume={184}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.184.4.1519}, DOI={10.1084/jem.184.4.1519}, number={4}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={McInnes, I B and Leung, B P and Field, M and Wei, X Q and Huang, F P and Sturrock, R D and Kinninmonth, A and Weidner, J and Mumford, R and Liew, F Y}, year={1996}, month=oct, pages={1519–1524} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        21
      ]
    ],
    "date-time": "2025-05-21T08:26:31Z",
    "timestamp": 1747815991811
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "4",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        10,
        1
      ]
    ]
  },
  "abstract": "<jats:p>We have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (approximately 90%) were NSE- and CD68-, with only a minor population (approximately 10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)-alpha. These results suggest that NO may mediate pathology in RA through the induction of TNF-alpha production.</jats:p>",
  "DOI": "10.1084/jem.184.4.1519",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        6,
        24
      ]
    ],
    "date-time": "2004-06-24T07:56:10Z",
    "timestamp": 1088063770000
  },
  "page": "1519-1524",
  "update-policy": "http://dx.doi.org/10.1084/jem.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 222,
  "title": "Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients.",
  "prefix": "10.1084",
  "volume": "184",
  "author": [
    {
      "given": "I B",
      "family": "McInnes",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "B P",
      "family": "Leung",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "M",
      "family": "Field",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "X Q",
      "family": "Wei",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "F P",
      "family": "Huang",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "R D",
      "family": "Sturrock",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "A",
      "family": "Kinninmonth",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "J",
      "family": "Weidner",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "R",
      "family": "Mumford",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    },
    {
      "given": "F Y",
      "family": "Liew",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunology, University of Glasgow, United Kingdom."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1996,
        10,
        1
      ]
    ]
  },
  "container-title": "The Journal of experimental medicine",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jem/article-pdf/184/4/1519/1679399/1519.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jem/article-pdf/184/4/1519/1679399/1519.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        25
      ]
    ],
    "date-time": "2023-07-25T07:27:04Z",
    "timestamp": 1690270024000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jem/article/184/4/1519/51158/Production-of-nitric-oxide-in-the-synovial"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        10,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1996,
          10,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1084/jem.184.4.1519",
  "relation": {},
  "ISSN": [
    "0022-1007",
    "1540-9538"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1996,
        10,
        1
      ]
    ]
  }
}