DOI: 10.1084/jem.183.5.2367. Differentiation of follicular dendritic cells and full antibody responses require tumor necrosis factor receptor-1 signaling.

Differentiation of follicular dendritic cells and full antibody responses require tumor necrosis factor receptor-1 signaling.

10.1084/jem.183.5.2367

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

Using mice double deficient for tumor necrosis factor (TNF) and lymphotoxin alpha (LT alpha), we demonstrated that TNF and/or LT alpha are necessary for development of a normal splenic microarchitecture and for isotype switch after immunization with sheep red blood cells (SRBC). In the present study, we extended these observations by determining which TNF receptor (TNFR) is involved in morphological and functional differentiation of the spleen. Spleen morphology and antibody response were investigated in wild-type, TNFR1-/-, TNFR2-/- and TNF/LT alpha-/- mice immunized with SRBC. TNF/LT alpha-/- mice, which have a complete disruption of the TNF/LT alpha signaling system including the LT beta-receptor pathway, displayed an abnormal microarchitecture, and isotype switch did not take place. TNFR1-/- and TNFR2-/- mice displayed a normal spleen microarchitecture and mounted an IgM and IgG antibody response to SRBC. However, the IgG production in TNFR1-/- mice was minimal, with citers leveling off 6 d after immunization. In this strain, immunofluorescence revealed a lack of follicular dendritic cells (FDC) network, detected with FDC-M1 as well as anti-CR1, and a lack of germinal centers, detected with peanut agglutinin. In conclusion, whereas normal splenic microarchitecture and isotype switch might require the LT beta receptor, differentiation of FDC network, development of germinal centers, and full IgG response depend on signaling via TNFR1.

Bibliography

Le Hir, M., Bluethmann, H., Kosco-Vilbois, M. H., MÃ¼ller, M., di Padova, F., Moore, M., Ryffel, B., & Eugster, H. P. (1996). Differentiation of follicular dendritic cells and full antibody responses require tumor necrosis factor receptor-1 signaling. The Journal of Experimental Medicine, 183(5), 2367â2372.

Authors 8

M Le Hir (first)
H Bluethmann (additional)
M H Kosco-Vilbois (additional)
M Müller (additional)
F di Padova (additional)
M Moore (additional)
B Ryffel (additional)
H P Eugster (additional)

References 0 Referenced 164

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 3:28 a.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 3:04 p.m.)
Issued	29 years, 4 months ago (May 1, 1996)
Published	29 years, 4 months ago (May 1, 1996)
Published Online	29 years, 4 months ago (May 1, 1996)
Published Print	29 years, 4 months ago (May 1, 1996)

Funders 0

None

BibTeX

@article{Le_Hir_1996, title={Differentiation of follicular dendritic cells and full antibody responses require tumor necrosis factor receptor-1 signaling.}, volume={183}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.183.5.2367}, DOI={10.1084/jem.183.5.2367}, number={5}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Le Hir, M and Bluethmann, H and Kosco-Vilbois, M H and Müller, M and di Padova, F and Moore, M and Ryffel, B and Eugster, H P}, year={1996}, month=may, pages={2367–2372} }

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