DOI: 10.1084/jem.180.6.2017. Interleukin 1 alpha activates two forms of p54 alpha mitogen-activated protein kinase in rabbit liver.

Interleukin 1 alpha activates two forms of p54 alpha mitogen-activated protein kinase in rabbit liver.

10.1084/jem.180.6.2017

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

We have identified in rabbits two hepatic forms of T669 peptide kinases that are very strongly activated after systemic injection with the inflammatory cytokine interleukin 1 (IL-1). The T669 peptide contains a major phosphorylation site of the epidermal growth factor receptor, threonine 699 and is a substrate for mitogen-activated protein (MAP) kinases. The kinases were purified to homogeneity and corresponded to 50- and 55-kD proteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid sequencing of 12 tryptic peptides of both kinases identified them as p54 MAP kinase alpha. This kinase belongs to the novel family of stress-activated protein kinases. This is the first evidence of IL-1 activating a specific protein kinase in vivo.

Bibliography

Kracht, M., Truong, O., Totty, N. F., Shiroo, M., & Saklatvala, J. (1994). Interleukin 1 alpha activates two forms of p54 alpha mitogen-activated protein kinase in rabbit liver. The Journal of Experimental Medicine, 180(6), 2017â2025.

Authors 5

M Kracht (first)
O Truong (additional)
N F Totty (additional)
M Shiroo (additional)
J Saklatvala (additional)

References 0 Referenced 43

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 2:28 a.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:26 a.m.)
Issued	30 years, 8 months ago (Dec. 1, 1994)
Published	30 years, 8 months ago (Dec. 1, 1994)
Published Online	30 years, 8 months ago (Dec. 1, 1994)
Published Print	30 years, 8 months ago (Dec. 1, 1994)

Funders 0

None

BibTeX

@article{Kracht_1994, title={Interleukin 1 alpha activates two forms of p54 alpha mitogen-activated protein kinase in rabbit liver.}, volume={180}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.180.6.2017}, DOI={10.1084/jem.180.6.2017}, number={6}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Kracht, M and Truong, O and Totty, N F and Shiroo, M and Saklatvala, J}, year={1994}, month=dec, pages={2017–2025} }

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