DOI: 10.1084/jem.179.2.533. Peptide length and sequence specificity of the mouse TAP1/TAP2 translocator.

Peptide length and sequence specificity of the mouse TAP1/TAP2 translocator.

10.1084/jem.179.2.533

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

The transporter associated with antigen processing (TAP) delivers peptides to the lumen of the endoplasmic reticulum in an adenosine triphosphate (ATP) dependent fashion for presentation by major histocompatibility complex class I molecules. We show that the mouse TAP translocator (H-2b haplotype) selects peptides based on a minimal size of nine residues, and on the presence of a hydrophobic COOH-terminal amino acid. The preponderance of COOH-terminal hydrophobic amino acids in peptides capable of binding to mouse class I molecules thus fits remarkably well with the specificity of the TAP translocator. In addition to transport in the lumenal direction, efflux of peptide in the cytosolic direction is observed in an ATP- and temperature-dependent manner. By maintaining a low peptide concentration at the site of class I assembly, this efflux mechanism may ensure that class I molecules are loaded preferentially with high affinity peptides.

Bibliography

Schumacher, T. N., Kantesaria, D. V., Heemels, M. T., Ashton-Rickardt, P. G., Shepherd, J. C., Fruh, K., Yang, Y., Peterson, P. A., Tonegawa, S., & Ploegh, H. L. (1994). Peptide length and sequence specificity of the mouse TAP1/TAP2 translocator. The Journal of Experimental Medicine, 179(2), 533â540.

Authors 10

T N Schumacher (first)
D V Kantesaria (additional)
M T Heemels (additional)
P G Ashton-Rickardt (additional)
J C Shepherd (additional)
K Fruh (additional)
Y Yang (additional)
P A Peterson (additional)
S Tonegawa (additional)
H L Ploegh (additional)

References 0 Referenced 161

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 2:02 a.m.)
Indexed	2 months ago (June 19, 2025, 7:24 a.m.)
Issued	31 years, 6 months ago (Feb. 1, 1994)
Published	31 years, 6 months ago (Feb. 1, 1994)
Published Online	31 years, 6 months ago (Feb. 1, 1994)
Published Print	31 years, 6 months ago (Feb. 1, 1994)

Funders 0

None

BibTeX

@article{Schumacher_1994, title={Peptide length and sequence specificity of the mouse TAP1/TAP2 translocator.}, volume={179}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.179.2.533}, DOI={10.1084/jem.179.2.533}, number={2}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Schumacher, T N and Kantesaria, D V and Heemels, M T and Ashton-Rickardt, P G and Shepherd, J C and Fruh, K and Yang, Y and Peterson, P A and Tonegawa, S and Ploegh, H L}, year={1994}, month=feb, pages={533–540} }

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