DOI: 10.1084/jem.177.5.1231. Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.

Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.

10.1084/jem.177.5.1231

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

Previously we showed that motility-related protein (MRP-1) is an antigen recognized by monoclonal antibody (mAb) M31-15 inhibiting cell motility and that the sequence of MRP-1 coincides with that of CD9. In the present study, plasmid was constructed in which human MRP-1/CD9 cDNA is expressed under the control of the Abelson murine leukemia virus promoter sequence. The expression plasmid for MRP-1/CD9 was introduced into Chinese hamster ovary cells, human lung adenocarcinoma cell line MAC10 (MRP-1 positive), and human myeloma cell line ARH77 (MRP-1 negative). All of the MRP-1/CD9 (over)expressing clones obtained from these transfected cells showed suppressed cell motility (penetration and phagokinetic track assays) depending on the degree of expression of MRP-1/CD9. Overexpression of MRP-1/CD9 by MAC10 cells resulted in the suppression of cell motility (maximally 73%) associated with considerable inhibition of the cell growth (maximally 48%). However, the inhibition of the growth of MAC10 cells by mAb M31-15 was &lt; 17% at an antibody concentration of 1-5 micrograms/ml, which inhibits cell motility by &gt; 90%. These results suggest that MRP-1/CD9 directly regulates cell motility and may also affect cell growth. Effects on metastasis by the expression of MRP-1 CD9 were investigated with mouse melanoma BL6 cells-BALB/c nu/nu mouse system. Metastatic potential of all transformants expressing MRP-1/CD9 was lower than that of parent BL6 cells.

Bibliography

Ikeyama, S., Koyama, M., Yamaoko, M., Sasada, R., & Miyake, M. (1993). Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA. The Journal of Experimental Medicine, 177(5), 1231â1237.

Authors 5

S Ikeyama (first)
M Koyama (additional)
M Yamaoko (additional)
R Sasada (additional)
M Miyake (additional)

References 0 Referenced 223

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 12:58 a.m.)
Indexed	4 months, 3 weeks ago (April 6, 2025, 12:47 p.m.)
Issued	32 years, 3 months ago (May 1, 1993)
Published	32 years, 3 months ago (May 1, 1993)
Published Online	32 years, 3 months ago (May 1, 1993)
Published Print	32 years, 3 months ago (May 1, 1993)

Funders 0

None

BibTeX

@article{Ikeyama_1993, title={Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.}, volume={177}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.177.5.1231}, DOI={10.1084/jem.177.5.1231}, number={5}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Ikeyama, S and Koyama, M and Yamaoko, M and Sasada, R and Miyake, M}, year={1993}, month=may, pages={1231–1237} }

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