DOI: 10.1084/jem.175.6.1443. Rhodamine123 reveals heterogeneity within murine Lin-, Sca-1+ hemopoietic stem cells.

Rhodamine123 reveals heterogeneity within murine Lin-, Sca-1+ hemopoietic stem cells.

10.1084/jem.175.6.1443

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

Murine bone marrow Lin-, Ly6A/E+ cells have been fractionated on the basis of rhodamine123 retention into Rh123med/hi and Rh123lo subpopulations. These populations have different responses to hemopoietic growth factors with respect to in vitro colony formation. Cells from either fraction were not stimulated by only granulocyte-colony-stimulating factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF), interleukins 1 and 6 (IL-1 and -6), or leukemia inhibitory factor (LIF) alone. The Rh123med/hi, but not the Rh123lo fraction, contained cells that could be stimulated by either stem cell factor (SCF) or IL-3 alone. When combinations of growth factors were added, the Rh123med/hi fraction produced more colonies, and responded to a wider range of factor combinations than the Rh123lo population. When tested in vivo, both populations contained no detectable day 8 colony-forming unit-spleen (CFU-S), and similar frequencies of day 13 CFU-S. When transplanted into lethally irradiated recipients (100 cells/recipient), significant numbers of donor cells (67-73%) were found in the peripheral blood of Rh123lo recipients. Both myeloid and lymphoid cells were of donor origin. By comparison, the Rh123med/hi population produced recipients with 1-2% donor cells in peripheral blood, the majority of which were lymphoid.

Bibliography

Li, C. L., & Johnson, G. R. (1992). Rhodamine123 reveals heterogeneity within murine Lin-, Sca-1+ hemopoietic stem cells. The Journal of Experimental Medicine, 175(6), 1443â1447.

Authors 2

C L Li (first)
G R Johnson (additional)

References 0 Referenced 98

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 12:46 a.m.)
Indexed	1 year ago (Aug. 12, 2024, 5:07 a.m.)
Issued	33 years, 2 months ago (June 1, 1992)
Published	33 years, 2 months ago (June 1, 1992)
Published Online	33 years, 2 months ago (June 1, 1992)
Published Print	33 years, 2 months ago (June 1, 1992)

Funders 0

None

BibTeX

@article{Li_1992, title={Rhodamine123 reveals heterogeneity within murine Lin-, Sca-1+ hemopoietic stem cells.}, volume={175}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.175.6.1443}, DOI={10.1084/jem.175.6.1443}, number={6}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Li, C L and Johnson, G R}, year={1992}, month=jun, pages={1443–1447} }

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