DOI: 10.1084/jem.174.1.21. In vitro proliferation and cloning of CD3- CD16+ cells from human thymocyte precursors.

In vitro proliferation and cloning of CD3- CD16+ cells from human thymocyte precursors.

10.1084/jem.174.1.21

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

Purified CD3-4- thymocytes were obtained by depletion of CD3+ and CD4+ cells from fresh thymocyte suspensions. 5-15% of these cells were found to express CD16 antigen, while other natural killer (NK) cell markers were virtually absent. Double fluorescence analysis revealed that 20-40% of thymic CD16+ cells coexpressed CD1, while approximately half were cyCD3+. When cultured in the presence of peripheral blood lymphocytes and H9 leukemia cell line as a source of irradiated feeder cells and interleukin 2 (IL-2), CD3-4- thymocytes underwent extensive proliferation. In addition, after 1-2 wk of culture, 30-50% of these cells were found to express CD16 surface antigen. Cloning under limiting dilution conditions of either CD3-4- or CD3-4-16- thymocytes in the presence of irradiated H9 cells resulted in large proportions (approximately 50%) of CD16+ clones. On the basis of the expression of surface CD16 and/or cyCD3 antigen, clones could be grouped in the following subsets: CD16+ cyCD3+; CD16+ cyCD3-; CD16- cyCD3+; and CD16- cyCD3-. All clones expressed CD56 surface antigen, displayed a strong cytolytic activity against NK sensitive (K562) and NK-resistant (M14) target cells, and produced IFN-gamma and tumor necrosis factor, but not IL-2. Similar to peripheral NK cells, thymic CD16+ cells expressed transcripts for CD16 and for CD3 epsilon (Biassoni, R., S. Ferrini, I. Prigione, A. Moretta, and E.O. Long, 1988. J. Immunol. 140:1685.) and zeta chains (Anderson, P., M. Caligiuri, J. Ritz, and S.F. Schlossman. 1989. Nature [Lond.]. 341:159). Therefore, it appears that cells that are phenotypically and functionally similar to CD3- CD16+ NK cells may arise from immature thymocytes.

Bibliography

Mingari, M. C., Poggi, A., Biassoni, R., Bellomo, R., Ciccone, E., Pella, N., Morelli, L., Verdiani, S., Moretta, A., & Moretta, L. (1991). In vitro proliferation and cloning of CD3- CD16+ cells from human thymocyte precursors. The Journal of Experimental Medicine, 174(1), 21â26.

Authors 10

M C Mingari (first)
A Poggi (additional)
R Biassoni (additional)
R Bellomo (additional)
E Ciccone (additional)
N Pella (additional)
L Morelli (additional)
S Verdiani (additional)
A Moretta (additional)
L Moretta (additional)

References 0 Referenced 60

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 9:59 p.m.)
Indexed	1 year, 1 month ago (July 15, 2024, 2:38 a.m.)
Issued	34 years, 2 months ago (July 1, 1991)
Published	34 years, 2 months ago (July 1, 1991)
Published Online	34 years, 2 months ago (July 1, 1991)
Published Print	34 years, 2 months ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Mingari_1991, title={In vitro proliferation and cloning of CD3- CD16+ cells from human thymocyte precursors.}, volume={174}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.174.1.21}, DOI={10.1084/jem.174.1.21}, number={1}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Mingari, M C and Poggi, A and Biassoni, R and Bellomo, R and Ciccone, E and Pella, N and Morelli, L and Verdiani, S and Moretta, A and Moretta, L}, year={1991}, month=jul, pages={21–26} }

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