DOI: 10.1084/jem.163.4.1024. A recombinant 64 kilodalton protein of Mycobacterium bovis bacillus Calmette-Guerin specifically stimulates human T4 clones reactive to mycobacterial antigens.

A recombinant 64 kilodalton protein of Mycobacterium bovis bacillus Calmette-Guerin specifically stimulates human T4 clones reactive to mycobacterial antigens.

10.1084/jem.163.4.1024

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

A recombinant 64 kD protein of Mycobacterium bovis bacillus Calmette-Guerin (BCG) (antigen A), which amounted to approximately 2% of an E. coli lysate, was tested for its capacity to stimulate human T4 clones reactive to mycobacterial proteins. Two out of four crossreactive clones, established from a patient with tuberculoid leprosy, which could be stimulated by protein preparations of M. leprae and M. tuberculosis, and by particulate M. bovis BCG were also reactive to antigen A without further enrichment from E. coli lysate. In addition, BCG-reactive T cell clones from two of three healthy PPD+ donors reacted with antigen A. This finding shows that human T cell clones may be useful for probing gene-cloned proteins of potential value for vaccination against diseases where protection is mediated exclusively by T cells.

Bibliography

Emmrich, F., Thole, J., van Embden, J., & Kaufmann, S. H. (1986). A recombinant 64 kilodalton protein of Mycobacterium bovis bacillus Calmette-Guerin specifically stimulates human T4 clones reactive to mycobacterial antigens. The Journal of Experimental Medicine, 163(4), 1024â1029.

Authors 4

F Emmrich (first)
J Thole (additional)
J van Embden (additional)
S H Kaufmann (additional)

References 0 Referenced 115

None

Dates

Type	When
Created	21 years, 2 months ago (June 24, 2004, 3:56 a.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 9 p.m.)
Indexed	4 months, 4 weeks ago (April 8, 2025, 5:39 p.m.)
Issued	39 years, 5 months ago (April 1, 1986)
Published	39 years, 5 months ago (April 1, 1986)
Published Online	39 years, 5 months ago (April 1, 1986)
Published Print	39 years, 5 months ago (April 1, 1986)

Funders 0

None

BibTeX

@article{Emmrich_1986, title={A recombinant 64 kilodalton protein of Mycobacterium bovis bacillus Calmette-Guerin specifically stimulates human T4 clones reactive to mycobacterial antigens.}, volume={163}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.163.4.1024}, DOI={10.1084/jem.163.4.1024}, number={4}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Emmrich, F and Thole, J and van Embden, J and Kaufmann, S H}, year={1986}, month=apr, pages={1024–1029} }

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