DOI: 10.1084/jem.154.2.422. Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.

Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.

10.1084/jem.154.2.422

Crossref journal-article

Rockefeller University Press

The Journal of experimental medicine (291)

Abstract

Mouse T cell growth factor was purified from the serum-free conditioned medium of lectin-stimulated spleen cells. A 3,000-fold purification was achieved with a final yield of 12%. The purified protein, with an apparent Mr of 23,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, was active at concentrations of 4 x 10(-11) M, both in the T cell growth factor and T cell replacing factor assays. In addition, purified T cell growth factor alone was mitogenic for spleen cells from both nude and normal mice.

Bibliography

Granelli-Piperno, A., Vassalli, J. D., & Reich, E. (1981). Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity. The Journal of Experimental Medicine, 154(2), 422â431.

Authors 3

A Granelli-Piperno (first)
J D Vassalli (additional)
E Reich (additional)

References 0 Referenced 28

None

Dates

Type	When
Created	21 years, 2 months ago (June 23, 2004, 5 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 8:30 p.m.)
Indexed	1 day, 16 hours ago (Sept. 4, 2025, 9:43 a.m.)
Issued	44 years, 1 month ago (Aug. 1, 1981)
Published	44 years, 1 month ago (Aug. 1, 1981)
Published Online	44 years, 1 month ago (Aug. 1, 1981)
Published Print	44 years, 1 month ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{Granelli_Piperno_1981, title={Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.}, volume={154}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.154.2.422}, DOI={10.1084/jem.154.2.422}, number={2}, journal={The Journal of experimental medicine}, publisher={Rockefeller University Press}, author={Granelli-Piperno, A and Vassalli, J D and Reich, E}, year={1981}, month=aug, pages={422–431} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:43:21Z",
    "timestamp": 1756993401915
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "2",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        8,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Mouse T cell growth factor was purified from the serum-free conditioned medium of lectin-stimulated spleen cells. A 3,000-fold purification was achieved with a final yield of 12%. The purified protein, with an apparent Mr of 23,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis, was active at concentrations of 4 x 10(-11) M, both in the T cell growth factor and T cell replacing factor assays. In addition, purified T cell growth factor alone was mitogenic for spleen cells from both nude and normal mice.</jats:p>",
  "DOI": "10.1084/jem.154.2.422",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        6,
        23
      ]
    ],
    "date-time": "2004-06-23T21:00:50Z",
    "timestamp": 1088024450000
  },
  "page": "422-431",
  "update-policy": "http://dx.doi.org/10.1084/jem.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 28,
  "title": "Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity.",
  "prefix": "10.1084",
  "volume": "154",
  "author": [
    {
      "given": "A",
      "family": "Granelli-Piperno",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J D",
      "family": "Vassalli",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "E",
      "family": "Reich",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1981,
        8,
        1
      ]
    ]
  },
  "container-title": "The Journal of experimental medicine",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jem/article-pdf/154/2/422/1662741/422.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jem/article-pdf/154/2/422/1662741/422.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        25
      ]
    ],
    "date-time": "2023-07-25T00:30:43Z",
    "timestamp": 1690245043000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jem/article/154/2/422/22817/Purification-of-murine-T-cell-growth-factor-A"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        8,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1981,
          8,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1084/jem.154.2.422",
  "relation": {},
  "ISSN": [
    "0022-1007",
    "1540-9538"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1981,
        8,
        1
      ]
    ]
  }
}