DOI: 10.1084/jem.112.2.237. BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

10.1084/jem.112.2.237

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

The correlation between morphological and biochemical changes produced by the antigen-antibody reaction was studied in cultures of tissue monocytes taken from sensitized animals. The cells were grown under conditions which allowed collection of samples from the culture fluid as well as microscopic observation. Introduction of the antigen into the culture medium causes rapid release of a protease characterized by its susceptibility to sulfhydryl block and its optimum pH in the neutral range. Protease activation occurs simultaneously with morphological changes in the cytoplasm of the cultured cells. Delayed changes affecting the mitochondria and Golgi bodies appear after the peak of the proteolytic reaction and may be secondary to it. The gradual inactivation of the protease observed in the course of the antigen-antibody reaction will be discussed in a separate paper.

Bibliography

Hayashi, H., Tokuda, A., & Udaka, K. (1960). BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE. The Journal of Experimental Medicine, 112(2), 237â247.

Authors 3

Hideo Hayashi (first)
Akira Tokuda (additional)
Keiji Udaka (additional)

References 0 Referenced 33

None

Dates

Type	When
Created	21 years, 2 months ago (June 22, 2004, 4:51 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 2:50 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 5:28 p.m.)
Issued	65 years, 1 month ago (Aug. 1, 1960)
Published	65 years, 1 month ago (Aug. 1, 1960)
Published Online	65 years, 1 month ago (Aug. 1, 1960)
Published Print	65 years, 1 month ago (Aug. 1, 1960)

Funders 0

None

BibTeX

@article{Hayashi_1960, title={BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE}, volume={112}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.112.2.237}, DOI={10.1084/jem.112.2.237}, number={2}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Hayashi, Hideo and Tokuda, Akira and Udaka, Keiji}, year={1960}, month=aug, pages={237–247} }

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