DOI: 10.1083/jcb.99.1.327. Two enzymes involved in the synthesis of O-linked oligosaccharides are localized on membranes of different densities in mouse lymphoma BW5147 cells.

Two enzymes involved in the synthesis of O-linked oligosaccharides are localized on membranes of different densities in mouse lymphoma BW5147 cells.

10.1083/jcb.99.1.327

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Microsomal membranes from mouse lymphoma BW5147 cells were fractionated on a continuous sucrose gradient and assayed for two enzymes involved in the synthesis of O-linked oligosaccharides. Both enzymes were recovered in membranes that were less dense than the membranes containing the endoplasmic reticulum marker enzymes, glucosidase I and II. UDP-Gal:N-acetylgalactosamine-beta 1, 3-galactosyltransferase had a distribution that coincided with that of the galactosyltransferase that acts on asparagine-linked oligosaccharides. This latter enzyme has been immunolocalized to the trans Golgi elements. The UDP-GalNAc:polypeptide N-acetylgalactosaminyl-transferase was recovered in a membrane fraction of intermediate density, between the endoplasmic reticulum and trans Golgi markers. These findings are consistent with the assembly of O-linked oligosaccharides occurring in at least two different Golgi compartments.

Bibliography

Elhammer, A., & Kornfeld, S. (1984). Two enzymes involved in the synthesis of O-linked oligosaccharides are localized on membranes of different densities in mouse lymphoma BW5147 cells. The Journal of Cell Biology, 99(1), 327â331.

Authors 2

A Elhammer (first)
S Kornfeld (additional)

References 0 Referenced 67

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 1:33 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 7, 2025, 4:53 a.m.)
Issued	41 years, 1 month ago (July 1, 1984)
Published	41 years, 1 month ago (July 1, 1984)
Published Online	41 years, 1 month ago (July 1, 1984)
Published Print	41 years, 1 month ago (July 1, 1984)

Funders 0

None

BibTeX

@article{Elhammer_1984, title={Two enzymes involved in the synthesis of O-linked oligosaccharides are localized on membranes of different densities in mouse lymphoma BW5147 cells.}, volume={99}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.99.1.327}, DOI={10.1083/jcb.99.1.327}, number={1}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Elhammer, A and Kornfeld, S}, year={1984}, month=jul, pages={327–331} }

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