DOI: 10.1083/jcb.96.3.717. The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.

The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.

10.1083/jcb.96.3.717

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A).

Bibliography

Baer, B. W., & Kornberg, R. D. (1983). The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein. The Journal of Cell Biology, 96(3), 717â721.

Authors 2

B W Baer (first)
R D Kornberg (additional)

References 0 Referenced 144

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 2:37 p.m.)
Indexed	1 month ago (July 20, 2025, 12:17 a.m.)
Issued	42 years, 5 months ago (March 1, 1983)
Published	42 years, 5 months ago (March 1, 1983)
Published Online	42 years, 5 months ago (March 1, 1983)
Published Print	42 years, 5 months ago (March 1, 1983)

Funders 0

None

BibTeX

@article{Baer_1983, title={The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.}, volume={96}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.96.3.717}, DOI={10.1083/jcb.96.3.717}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Baer, B W and Kornberg, R D}, year={1983}, month=mar, pages={717–721} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        20
      ]
    ],
    "date-time": "2025-07-20T04:17:09Z",
    "timestamp": 1752985029506
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "3",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1983,
        3,
        1
      ]
    ]
  },
  "abstract": "<jats:p>A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A).</jats:p>",
  "DOI": "10.1083/jcb.96.3.717",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        14
      ]
    ],
    "date-time": "2004-05-14T23:04:59Z",
    "timestamp": 1084575899000
  },
  "page": "717-721",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 144,
  "title": "The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.",
  "prefix": "10.1083",
  "volume": "96",
  "author": [
    {
      "given": "B W",
      "family": "Baer",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "R D",
      "family": "Kornberg",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1983,
        3,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/96/3/717/1646559/717.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/96/3/717/1646559/717.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        24
      ]
    ],
    "date-time": "2023-07-24T18:37:30Z",
    "timestamp": 1690223850000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/96/3/717/29903/The-protein-responsible-for-the-repeating"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1983,
        3,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1983,
          3,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.96.3.717",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1983,
        3,
        1
      ]
    ]
  }
}