DOI: 10.1083/jcb.94.3.592. Visualization of a 21-nm axial periodicity in shadowed keratin filaments and neurofilaments.

Visualization of a 21-nm axial periodicity in shadowed keratin filaments and neurofilaments.

10.1083/jcb.94.3.592

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Unidirectional and rotary shadowing techniques have been applied in studying the surface structure of two types of intermediate filaments. Keratin filaments and neurofilaments demonstrate a approximately 21-nm axial periodicity which probably indicates the helical pitch of the outer shell of the filament. Analysis of unidirectionally shadowed keratin showed that the helix is left-handed. The observation of a left-handed helix of 21-nm pitch supports the three-stranded protofilament model of Fraser, Macrae, and Suzuki (1976, J. Mol. Biol. 108:435-452), and indicates that keratin filaments probably consist of 10 three-stranded protofilaments surrounding a core of three such protofilaments, as predicted by models based on x-ray diffraction of hard keratin filaments. Neurofilaments do not demonstrate an easily identifiable hand, so their consistency with the model is, as yet, uncertain.

Bibliography

Milam, L., & Erickson, H. P. (1982). Visualization of a 21-nm axial periodicity in shadowed keratin filaments and neurofilaments. The Journal of Cell Biology, 94(3), 592â596.

Authors 2

L Milam (first)
H P Erickson (additional)

References 0 Referenced 72

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years ago (July 24, 2023, 12:58 p.m.)
Indexed	1 year, 2 months ago (June 18, 2024, 7:57 a.m.)
Issued	42 years, 11 months ago (Sept. 1, 1982)
Published	42 years, 11 months ago (Sept. 1, 1982)
Published Online	42 years, 11 months ago (Sept. 1, 1982)
Published Print	42 years, 11 months ago (Sept. 1, 1982)

Funders 0

None

BibTeX

@article{Milam_1982, title={Visualization of a 21-nm axial periodicity in shadowed keratin filaments and neurofilaments.}, volume={94}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.94.3.592}, DOI={10.1083/jcb.94.3.592}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Milam, L and Erickson, H P}, year={1982}, month=sep, pages={592–596} }

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