DOI: 10.1083/jcb.88.2.257. Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.

Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.

10.1083/jcb.88.2.257

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

X-ray microanalysis of frozen-hydrated tissue sections permits direct quantitative analysis of diffusible elements in defined cellular compartments. Because the sections are hydrated, elemental concentrations can be defined as wet-weight mass fractions. Use of these techniques should also permit determination of water fraction in cellular compartments. Reliable preparative techniques provide flat, smooth, 0.5 micrometers-thick sections with little elemental and morphological disruption. The specimen support and transfer system described permits hydrated sections to be transferred to the scanning electron microscope cold stage for examination and analysis without contamination or water loss and without introduction of extraneous x-ray radiation.

Bibliography

Saubermann, A. J., Echlin, P., Peters, P. D., & Beeuwkes, R. (1981). Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques. The Journal of Cell Biology, 88(2), 257â267.

Authors 4

A J Saubermann (first)
P Echlin (additional)
P D Peters (additional)
R Beeuwkes (additional)

References 0 Referenced 82

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 5:36 a.m.)
Indexed	1 year, 9 months ago (Nov. 14, 2023, 10:24 a.m.)
Issued	44 years, 6 months ago (Feb. 1, 1981)
Published	44 years, 6 months ago (Feb. 1, 1981)
Published Online	44 years, 6 months ago (Feb. 1, 1981)
Published Print	44 years, 6 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{Saubermann_1981, title={Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.}, volume={88}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.88.2.257}, DOI={10.1083/jcb.88.2.257}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Saubermann, A J and Echlin, P and Peters, P D and Beeuwkes, R}, year={1981}, month=feb, pages={257–267} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        14
      ]
    ],
    "date-time": "2023-11-14T15:24:41Z",
    "timestamp": 1699975481835
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "2",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:p>X-ray microanalysis of frozen-hydrated tissue sections permits direct quantitative analysis of diffusible elements in defined cellular compartments. Because the sections are hydrated, elemental concentrations can be defined as wet-weight mass fractions. Use of these techniques should also permit determination of water fraction in cellular compartments. Reliable preparative techniques provide flat, smooth, 0.5 micrometers-thick sections with little elemental and morphological disruption. The specimen support and transfer system described permits hydrated sections to be transferred to the scanning electron microscope cold stage for examination and analysis without contamination or water loss and without introduction of extraneous x-ray radiation.</jats:p>",
  "DOI": "10.1083/jcb.88.2.257",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        14
      ]
    ],
    "date-time": "2004-05-14T23:04:59Z",
    "timestamp": 1084575899000
  },
  "page": "257-267",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 82,
  "title": "Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.",
  "prefix": "10.1083",
  "volume": "88",
  "author": [
    {
      "given": "A J",
      "family": "Saubermann",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "P",
      "family": "Echlin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "P D",
      "family": "Peters",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "suffix": "3rd",
      "given": "R",
      "family": "Beeuwkes",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1981,
        2,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/88/2/257/1637926/257.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/88/2/257/1637926/257.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        24
      ]
    ],
    "date-time": "2023-07-24T09:36:58Z",
    "timestamp": 1690191418000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/88/2/257/20953/Application-of-scanning-electron-microscopy-to-x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        2,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1981,
          2,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.88.2.257",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1981,
        2,
        1
      ]
    ]
  }
}