DOI: 10.1083/jcb.78.2.r23. Neurofilament protein is phosphorylated in the squid giant axon.

Neurofilament protein is phosphorylated in the squid giant axon.

10.1083/jcb.78.2.r23

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have observed the phosphorylation of neurofilament protein from squid axoplasm. Phosphorylation is demonstrated by 32P labeling of protein during incubation of axoplasm with [gamma-32P]ATP. When the labeled proteins are separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), two bands, at 2.0 x 10(5) daltons and greater than 4 x 10(5) daltons, contain the bulk of the 32P. The 2.0 x 10(5)-dalton phosphorylated polypeptide comigrates on SDS-PAGE with one of the subunits of squid neurofilament protein. Both major phosphorylated polypeptides co-fractionate with neurofilaments in discontinuous sucrose gradient centrifugation and on gel filtration chromatography on Sepharose 4B. The protein-phosphate bond behaves like a phospho-ester, and labeled phospho-serine is identified in an acid hydrolysate of the protein. The generality of this phenomenon in various species and its possible physiological significance are discussed.

Bibliography

Pant, H. C., Shecket, G., Gainer, H., & Lasek, R. J. (1978). Neurofilament protein is phosphorylated in the squid giant axon. The Journal of Cell Biology, 78(2), R23.

Authors 4

H C Pant (first)
G Shecket (additional)
H Gainer (additional)
R J Lasek (additional)

References 0 Referenced 63

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years ago (July 24, 2023, 4:32 a.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:02 a.m.)
Issued	47 years ago (Aug. 1, 1978)
Published	47 years ago (Aug. 1, 1978)
Published Online	47 years ago (Aug. 1, 1978)
Published Print	47 years ago (Aug. 1, 1978)

Funders 0

None

BibTeX

@article{Pant_1978, title={Neurofilament protein is phosphorylated in the squid giant axon.}, volume={78}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.78.2.r23}, DOI={10.1083/jcb.78.2.r23}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Pant, H C and Shecket, G and Gainer, H and Lasek, R J}, year={1978}, month=aug, pages={R23} }

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