DOI: 10.1083/jcb.75.1.23. Freeze-fracture of membrane fusions during exocytosis in pancreatic B-cells.

Freeze-fracture of membrane fusions during exocytosis in pancreatic B-cells.

10.1083/jcb.75.1.23

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

To examine the freeze-fracture appearance of membrane alterations at sites of exocytosis in mammalian cells, we studied the secretory granule and plasma membrane of rat pancreatic B-cells during glucose-stimulated insulin secretion. Constant features observed were the scarcity of particles in secretory-granule P-fracture faces and the almost total clearance of intramembranous particles in P-and E fracture faces of the plasma membrane in areas of close apposition of these two membranes preceding fusion; also observed was the temporary persistence of particle-cleared regions after the fusion was completed. Our observations thus support the concept that membranes fuse at sites of closely apposed, particle-free regions and that the physiologically created clear areas found in freeze-fracture replicas of the plasma membrane are the hallmarks of incipient or recent membrane fusion.

Bibliography

Orci, L., Perrelet, A., & Friend, D. S. (1977). Freeze-fracture of membrane fusions during exocytosis in pancreatic B-cells. The Journal of Cell Biology, 75(1), 23â30.

Authors 3

L Orci (first)
A Perrelet (additional)
D S Friend (additional)

References 0 Referenced 101

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 3:58 a.m.)
Indexed	1 year ago (Aug. 9, 2024, 9:38 p.m.)
Issued	47 years, 11 months ago (Oct. 1, 1977)
Published	47 years, 11 months ago (Oct. 1, 1977)
Published Online	47 years, 11 months ago (Oct. 1, 1977)
Published Print	47 years, 11 months ago (Oct. 1, 1977)

Funders 0

None

BibTeX

@article{Orci_1977, title={Freeze-fracture of membrane fusions during exocytosis in pancreatic B-cells.}, volume={75}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.75.1.23}, DOI={10.1083/jcb.75.1.23}, number={1}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Orci, L and Perrelet, A and Friend, D S}, year={1977}, month=oct, pages={23–30} }

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