DOI: 10.1083/jcb.74.3.940. Characterization of the myosin-phosphorylating system in normal murine astrocytes and derivative sv40 wild-type and A-mutant transformant.

Characterization of the myosin-phosphorylating system in normal murine astrocytes and derivative sv40 wild-type and A-mutant transformant.

10.1083/jcb.74.3.940

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Myosin and myosin light-chain kinase have been isolated and characterized from small quantities of normal and SV40-transformed, murine astrocytic neuroglial cells in culture and from intact normal mouse brain. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the astrocyte myosins revealed a heavy chain of 200,000 daltons and two light chains of 20,000 and 15,000 daltons. These myosins are similar to other cytyplasmic myosins. The astrocyte 20,000-dalton light chain can be phosphorylated by an endogenous myosin light-chain kinase which has properties similar to those of the myosin light-chain kinase found in human platelets. No differences were detected in either the astrocyte myosins or myosin light-chain kinases between (a) the normal and transformed cells, (b) the transformed cells grown at the permissive and nonpermissive temperatures, or (c) the SV40 wild-type and A-mutant transformants.

Bibliography

Scrodilis, S. P., Anderson, J. L., Pollack, R., & Adelstein, R. S. (1977). Characterization of the myosin-phosphorylating system in normal murine astrocytes and derivative sv40 wild-type and A-mutant transformant. The Journal of Cell Biology, 74(3), 940â949.

Authors 4

S P Scrodilis (first)
J L Anderson (additional)
R Pollack (additional)
R S Adelstein (additional)

References 0 Referenced 22

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 4:01 a.m.)
Indexed	1 year, 10 months ago (Oct. 19, 2023, 9:24 p.m.)
Issued	48 years ago (Sept. 1, 1977)
Published	48 years ago (Sept. 1, 1977)
Published Online	48 years ago (Sept. 1, 1977)
Published Print	48 years ago (Sept. 1, 1977)

Funders 0

None

BibTeX

@article{Scrodilis_1977, title={Characterization of the myosin-phosphorylating system in normal murine astrocytes and derivative sv40 wild-type and A-mutant transformant.}, volume={74}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.74.3.940}, DOI={10.1083/jcb.74.3.940}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Scrodilis, S P and Anderson, J L and Pollack, R and Adelstein, R S}, year={1977}, month=sep, pages={940–949} }

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