DOI: 10.1083/jcb.74.2.341. A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues.

A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues.

10.1083/jcb.74.2.341

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

A rapid method for measuring polymerized and depolymerized forms of tubulin in tissues has been developed. The procedure consists of homogenization and centrifugation of the tissue in a microtubule-stabilizing solution and depolymerization of the precipitated microtubules; polymerized and depolymerized forms of tubulin are quantitated by a colchicine-binding assay. The validity of the technique was assessed by electron microscopy and recovery studies with labeled and unlabeled preparations of polymerized and depolymerized forms of rat brain tubulin. The sensitivity of this technique allows quantitation of tubulin in 150 micrograms of tissue, wet weight. The method demonstrated that both the polymerized and depolymerized forms of tubulin were present in rat liver cells, and that in the fed state 31.3 +/-0.7% of the total tubulin pool was in the polymerized form.

Bibliography

Pipeleers, D. G., Pipeleers-Marichal, M. A., Sherline, P., & Kipnis, D. M. (1977). A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues. The Journal of Cell Biology, 74(2), 341â350.

Authors 4

D G Pipeleers (first)
M A Pipeleers-Marichal (additional)
P Sherline (additional)
D M Kipnis (additional)

References 0 Referenced 71

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 3:51 a.m.)
Indexed	3 months, 3 weeks ago (May 4, 2025, 5:22 p.m.)
Issued	48 years, 1 month ago (Aug. 1, 1977)
Published	48 years, 1 month ago (Aug. 1, 1977)
Published Online	48 years, 1 month ago (Aug. 1, 1977)
Published Print	48 years, 1 month ago (Aug. 1, 1977)

Funders 0

None

BibTeX

@article{Pipeleers_1977, title={A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues.}, volume={74}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.74.2.341}, DOI={10.1083/jcb.74.2.341}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Pipeleers, D G and Pipeleers-Marichal, M A and Sherline, P and Kipnis, D M}, year={1977}, month=aug, pages={341–350} }

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