DOI: 10.1083/jcb.71.3.894. Improved procedures for immunoferritin labeling of ultrathin frozen sections.

Improved procedures for immunoferritin labeling of ultrathin frozen sections.

10.1083/jcb.71.3.894

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross-linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems.

Bibliography

Tokuyasu, K. T., & Singer, S. J. (1976). Improved procedures for immunoferritin labeling of ultrathin frozen sections. The Journal of Cell Biology, 71(3), 894â906.

Authors 2

K T Tokuyasu (first)
S J Singer (additional)

References 0 Referenced 189

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years ago (July 24, 2023, 3:41 a.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:27 a.m.)
Issued	48 years, 8 months ago (Dec. 1, 1976)
Published	48 years, 8 months ago (Dec. 1, 1976)
Published Online	48 years, 8 months ago (Dec. 1, 1976)
Published Print	48 years, 8 months ago (Dec. 1, 1976)

Funders 0

None

BibTeX

@article{Tokuyasu_1976, title={Improved procedures for immunoferritin labeling of ultrathin frozen sections.}, volume={71}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.71.3.894}, DOI={10.1083/jcb.71.3.894}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Tokuyasu, K T and Singer, S J}, year={1976}, month=dec, pages={894–906} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        7
      ]
    ],
    "date-time": "2025-07-07T06:27:58Z",
    "timestamp": 1751869678989
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "3",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1976,
        12,
        1
      ]
    ]
  },
  "abstract": "<jats:p>In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross-linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems.</jats:p>",
  "DOI": "10.1083/jcb.71.3.894",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        14
      ]
    ],
    "date-time": "2004-05-14T23:04:59Z",
    "timestamp": 1084575899000
  },
  "page": "894-906",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 189,
  "title": "Improved procedures for immunoferritin labeling of ultrathin frozen sections.",
  "prefix": "10.1083",
  "volume": "71",
  "author": [
    {
      "given": "K T",
      "family": "Tokuyasu",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "S J",
      "family": "Singer",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1976,
        12,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/71/3/894/1632618/894.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/71/3/894/1632618/894.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        24
      ]
    ],
    "date-time": "2023-07-24T07:41:44Z",
    "timestamp": 1690184504000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/71/3/894/77804/Improved-procedures-for-immunoferritin-labeling-of"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1976,
        12,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1976,
          12,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.71.3.894",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1976,
        12,
        1
      ]
    ]
  }
}