Crossref journal-article
Rockefeller University Press
The Journal of cell biology (291)
Abstract

The distribution of (Na+ + K+) ATPase over the plasma membranes of the proximal convoluted tubule from canine renal cortex has been determined. Ultrathin frozen sections of this tissue were stained with rabbit antibodies to this enzyme and ferritin-conjugated goat antirabbit gamma-globulin. It is demonstrated that high concentrations of this enzyme uniformly line the intercellular spaces of this epithelium. The consequences of this observation are discussed in terms of the low resistant tight junctions of these tubules and the isotonic fluid transport which they support. Furthermore, antibodies to (Na+ + K+) ATPase recognize an antigen on the luminal surfaces of the tubules within the brush border. It is proposed that the enzyme is present in this region of the plasma membrane as well, although at much lower concentration. To further substantiate this conclusion, a brush border fraction has been purified from rabbit kidney and been shown to contain significant (Na+ + K+) ATPase. These results contradict earlier conclusions about the location of (Na+ + K+) ATPase in this tissue.

Bibliography

Kyte, J. (1976). Immunoferritin determination of the distribution of (Na+ + K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment. The Journal of Cell Biology, 68(2), 304–318.

Authors 1
  1. J Kyte (first)
References 0 Referenced 81

None

Dates
Type When
Created 21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited 2 years ago (July 24, 2023, 3:16 a.m.)
Indexed 1 year, 9 months ago (Nov. 11, 2023, 12:17 p.m.)
Issued 49 years, 6 months ago (Feb. 1, 1976)
Published 49 years, 6 months ago (Feb. 1, 1976)
Published Online 49 years, 6 months ago (Feb. 1, 1976)
Published Print 49 years, 6 months ago (Feb. 1, 1976)
Funders 0

None

@article{Kyte_1976, title={Immunoferritin determination of the distribution of (Na+ + K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment.}, volume={68}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.68.2.304}, DOI={10.1083/jcb.68.2.304}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Kyte, J}, year={1976}, month=feb, pages={304–318} }