DOI: 10.1083/jcb.66.1.188. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

10.1083/jcb.66.1.188

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.

Bibliography

Krishan, A. (1975). Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. The Journal of Cell Biology, 66(1), 188â193.

Authors 1

A Krishan (first)

References 0 Referenced 1,288

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 2:44 a.m.)
Indexed	4 days, 8 hours ago (Aug. 21, 2025, 1:38 p.m.)
Issued	50 years, 1 month ago (July 1, 1975)
Published	50 years, 1 month ago (July 1, 1975)
Published Online	50 years, 1 month ago (July 1, 1975)
Published Print	50 years, 1 month ago (July 1, 1975)

Funders 0

None

BibTeX

@article{Krishan_1975, title={Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.}, volume={66}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.66.1.188}, DOI={10.1083/jcb.66.1.188}, number={1}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Krishan, A}, year={1975}, month=jul, pages={188–193} }

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