DOI: 10.1083/jcb.64.2.331. Two improved methods for preparing ferritin-protein conjugates for electron microscopy.

Two improved methods for preparing ferritin-protein conjugates for electron microscopy.

10.1083/jcb.64.2.331

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

Bibliography

Kishida, Y., Olsen, B. R., Berg, R. A., & Prockop, D. J. (1975). Two improved methods for preparing ferritin-protein conjugates for electron microscopy. The Journal of Cell Biology, 64(2), 331â339.

Authors 4

Y Kishida (first)
B R Olsen (additional)
R A Berg (additional)
D J Prockop (additional)

References 0 Referenced 101

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 7:04 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 2:32 a.m.)
Indexed	1 year, 3 months ago (May 6, 2024, 3:53 p.m.)
Issued	50 years, 6 months ago (Feb. 1, 1975)
Published	50 years, 6 months ago (Feb. 1, 1975)
Published Online	50 years, 6 months ago (Feb. 1, 1975)
Published Print	50 years, 6 months ago (Feb. 1, 1975)

Funders 0

None

BibTeX

@article{Kishida_1975, title={Two improved methods for preparing ferritin-protein conjugates for electron microscopy.}, volume={64}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.64.2.331}, DOI={10.1083/jcb.64.2.331}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Kishida, Y and Olsen, B R and Berg, R A and Prockop, D J}, year={1975}, month=feb, pages={331–339} }

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