DOI: 10.1083/jcb.60.3.541. THE EFFECTS OF THROMBIN ON PHYTOHEMAGGLUTININ RECEPTOR SITES IN HUMAN PLATELETS

THE EFFECTS OF THROMBIN ON PHYTOHEMAGGLUTININ RECEPTOR SITES IN HUMAN PLATELETS

10.1083/jcb.60.3.541

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We have previously demonstrated that lentil phytohemagglutinin (lentil-PHA) binds to human platelet membranes without causing either aggregation or the release reaction. When platelets are treated with thrombin, there is an increase in lentil-PHA binding suggesting the appearance of new receptor sites on the cell surface. We prepared a lentil-PHA-ferritin conjugate using affinity chromatography which was used to saturate cell surface receptor sites. Studies using this conjugate suggest that thrombin causes a complex change in the platelet surface involving a decrease in the number of lentil-PHA receptor sites on the external platelet surface with a marked increase in sites within the center of the canalicular system. These increased sites may result from fusion of granule membranes with the canalicular membranes during the secretion process. There is no obvious relationship between lentil-PHA receptor sites and intramembranous particles.

Bibliography

Feagler, J. R., Tillack, T. W., Chaplin, D. D., & Majerus, P. W. (1974). THE EFFECTS OF THROMBIN ON PHYTOHEMAGGLUTININ RECEPTOR SITES IN HUMAN PLATELETS. The Journal of Cell Biology, 60(3), 541â553.

Authors 4

John R. Feagler (first)
Thomas W. Tillack (additional)
David D. Chaplin (additional)
Philip W. Majerus (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 24, 2023, 2:05 a.m.)
Indexed	1 year, 9 months ago (Nov. 11, 2023, 7:14 a.m.)
Issued	51 years, 6 months ago (March 1, 1974)
Published	51 years, 6 months ago (March 1, 1974)
Published Online	51 years, 6 months ago (March 1, 1974)
Published Print	51 years, 6 months ago (March 1, 1974)

Funders 0

None

BibTeX

@article{Feagler_1974, title={THE EFFECTS OF THROMBIN ON PHYTOHEMAGGLUTININ RECEPTOR SITES IN HUMAN PLATELETS}, volume={60}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.60.3.541}, DOI={10.1083/jcb.60.3.541}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Feagler, John R. and Tillack, Thomas W. and Chaplin, David D. and Majerus, Philip W.}, year={1974}, month=mar, pages={541–553} }

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