DOI: 10.1083/jcb.53.3.798. DETECTION OF DNA BY TRITIATED ACTINOMYCIN D ON ULTRATHIN FROZEN SECTIONS

DETECTION OF DNA BY TRITIATED ACTINOMYCIN D ON ULTRATHIN FROZEN SECTIONS

10.1083/jcb.53.3.798

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Ultrathin frozen sections of fresh liver tissue were floated on actinomycin D-3H. Quantitative high resolution radioautography was performed to determine the value of the method for detection of DNA by electron microscopy. A complete series of control experiments involving various treatments of frozen sections with enzymes (pronase, DNase) and 0.1 N HCl were also carried out to determine the specificity of the labeling. The results indicate the value of the method for detection of DNA directly on ultrathin frozen sections. Short treatments with pronase followed by DNase reduce the labeling to zero, whereas removal of chromosomal proteins with HCl increases the amount of radioactivity in the nucleus considerably. The results are discussed in view of the future applications opened by ultracryotomy, since radioautographic detection of various macromolecules and cellular components by labeled compound with specific affinities will now be possible.

Bibliography

Bernier, R., Iglesias, R., & Simard, R. (1972). DETECTION OF DNA BY TRITIATED ACTINOMYCIN D ON ULTRATHIN FROZEN SECTIONS. The Journal of Cell Biology, 53(3), 798â808.

Authors 3

Roch Bernier (first)
Roberto Iglesias (additional)
René Simard (additional)

References 0 Referenced 14

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years ago (July 23, 2023, 11:33 p.m.)
Indexed	1 year, 9 months ago (Nov. 10, 2023, 3:44 p.m.)
Issued	53 years, 2 months ago (June 1, 1972)
Published	53 years, 2 months ago (June 1, 1972)
Published Online	53 years, 2 months ago (June 1, 1972)
Published Print	53 years, 2 months ago (June 1, 1972)

Funders 0

None

BibTeX

@article{Bernier_1972, title={DETECTION OF DNA BY TRITIATED ACTINOMYCIN D ON ULTRATHIN FROZEN SECTIONS}, volume={53}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.53.3.798}, DOI={10.1083/jcb.53.3.798}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bernier, Roch and Iglesias, Roberto and Simard, René}, year={1972}, month=jun, pages={798–808} }

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