DOI: 10.1083/jcb.53.1.116. FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

10.1083/jcb.53.1.116

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.

Bibliography

Plattner, H., Fischer, W. M., Schmitt, W. W., & Bachmann, L. (1972). FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS. The Journal of Cell Biology, 53(1), 116â126.

Authors 4

Helmut Plattner (first)
Walter M. Fischer (additional)
Werner W. Schmitt (additional)
Luis Bachmann (additional)

References 0 Referenced 56

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 11:32 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 4, 2024, 4:53 p.m.)
Issued	53 years, 4 months ago (April 1, 1972)
Published	53 years, 4 months ago (April 1, 1972)
Published Online	53 years, 4 months ago (April 1, 1972)
Published Print	53 years, 4 months ago (April 1, 1972)

Funders 0

None

BibTeX

@article{Plattner_1972, title={FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS}, volume={53}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.53.1.116}, DOI={10.1083/jcb.53.1.116}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Plattner, Helmut and Fischer, Walter M. and Schmitt, Werner W. and Bachmann, Luis}, year={1972}, month=apr, pages={116–126} }

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