DOI: 10.1083/jcb.51.2.525. AN ASSAY FOR INTERCELLULAR ADHESIVE SPECIFICITY

AN ASSAY FOR INTERCELLULAR ADHESIVE SPECIFICITY

10.1083/jcb.51.2.525

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A modification of an assay for intercellular adhesive specificity is described. The method involves the collection of radioactively labeled cells by aggregates of the same (isotypic aggregates) or different (heterotypic aggregates) types of tissue and determination of the number of cells collected by liquid scintillation counting. The use of 32P to label the tissues permitted a much more rapid estimation of cell collection than was obtained previously. With the use of chick embryo neural retina, liver, forebrain, pectoral muscle, and heart ventricle tissue, it was shown that isotypic was always greater than heterotypic collection. Labeled neural retina cell collection by neural retina aggregates was studied as a function of time, cell suspension density, aggregate diameter, temperature, and aggregate number. Neural retina aggregates were treated with certain enzymes in an attempt to determine whether specific changes on the surface of the aggregates would interfere with labeled neural retina cell collection. Of the various proteases and glycosidases tested, only ß-galactosidase rendered the surface more nonspecific.

Bibliography

Roth, S., McGuire, E. J., & Roseman, S. (1971). AN ASSAY FOR INTERCELLULAR ADHESIVE SPECIFICITY. The Journal of Cell Biology, 51(2), 525â535.

Authors 3

Stephen Roth (first)
Edward J. McGuire (additional)
Saul Roseman (additional)

References 0 Referenced 122

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 11:20 p.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 3:25 a.m.)
Issued	53 years, 9 months ago (Nov. 1, 1971)
Published	53 years, 9 months ago (Nov. 1, 1971)
Published Online	53 years, 9 months ago (Nov. 1, 1971)
Published Print	53 years, 9 months ago (Nov. 1, 1971)

Funders 0

None

BibTeX

@article{Roth_1971, title={AN ASSAY FOR INTERCELLULAR ADHESIVE SPECIFICITY}, volume={51}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.51.2.525}, DOI={10.1083/jcb.51.2.525}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Roth, Stephen and McGuire, Edward J. and Roseman, Saul}, year={1971}, month=nov, pages={525–535} }

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