DOI: 10.1083/jcb.51.1.265. THE LOCALIZATION OF SPECTRIN ON THE INNER SURFACE OF HUMAN RED BLOOD CELL MEMBRANES BY FERRITIN-CONJUGATED ANTIBODIES

THE LOCALIZATION OF SPECTRIN ON THE INNER SURFACE OF HUMAN RED BLOOD CELL MEMBRANES BY FERRITIN-CONJUGATED ANTIBODIES

10.1083/jcb.51.1.265

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Spectrin, a major protein constituent of mammalian red blood cell membrane preparations, has been localized on the inner surface of human red blood cell membranes by techniques that utilized specific ferritin-conjugated antibodies and fixation of membranes shortly after hemolysis so as to allow penetration of the ferritin-antibody labels. The labeling of spectrin was shown to be specific by the following criteria. (a) Nonhomologous ferritin-conjugated antibodies did not specifically bind to either membrane surface. (b) Blocking the membrane-bound spectrin with excess unconjugated antispectrin antibodies prevented ferritin-antibody labeling. (c) Removal of spectrin by treating the membrane preparation with a low ionic strength buffer containing ethylenediaminetetraacetate and ß-mercaptoethanol prevented labeling by specific ferritin-conjugated antibodies.

Bibliography

Nicolson, G. L., Marchesi, V. T., & Singer, S. J. (1971). THE LOCALIZATION OF SPECTRIN ON THE INNER SURFACE OF HUMAN RED BLOOD CELL MEMBRANES BY FERRITIN-CONJUGATED ANTIBODIES. The Journal of Cell Biology, 51(1), 265â272.

Authors 3

Garth L. Nicolson (first)
V. T. Marchesi (additional)
S. J. Singer (additional)

References 0 Referenced 142

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years ago (July 23, 2023, 11:18 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 7:16 a.m.)
Issued	53 years, 10 months ago (Oct. 1, 1971)
Published	53 years, 10 months ago (Oct. 1, 1971)
Published Online	53 years, 10 months ago (Oct. 1, 1971)
Published Print	53 years, 10 months ago (Oct. 1, 1971)

Funders 0

None

BibTeX

@article{Nicolson_1971, title={THE LOCALIZATION OF SPECTRIN ON THE INNER SURFACE OF HUMAN RED BLOOD CELL MEMBRANES BY FERRITIN-CONJUGATED ANTIBODIES}, volume={51}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.51.1.265}, DOI={10.1083/jcb.51.1.265}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Nicolson, Garth L. and Marchesi, V. T. and Singer, S. J.}, year={1971}, month=oct, pages={265–272} }

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