DOI: 10.1083/jcb.49.3.731. IMPROVED TECHNIQUES FOR THE PREPARATION OF ULTRATHIN FROZEN SECTIONS

IMPROVED TECHNIQUES FOR THE PREPARATION OF ULTRATHIN FROZEN SECTIONS

10.1083/jcb.49.3.731

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Ultrathin frozen sections of biological tissues for electron microscopy provide certain advantages in cytochemical studies in which the penetration of cells by large molecules is necessary and in morphological studies of cellular constituents which are dissolved by the reagents employed in routine plastic embedding. The recent introduction of several types of commercially available cryo-ultramicrotomes makes it possible for many laboratories to employ this valuable tool. This paper summarizes recent improvements in the methods developed in this laboratory for preparing ultrathin frozen sections and reviews some of the inherent problems involved in their use. These procedures may serve as a baseline for other investigators who can then modify or adapt them for their specific purposes.

Bibliography

Bernhard, W., & Viron, A. (1971). IMPROVED TECHNIQUES FOR THE PREPARATION OF ULTRATHIN FROZEN SECTIONS. The Journal of Cell Biology, 49(3), 731â746.

Authors 2

W. Bernhard (first)
Annie Viron (additional)

References 0 Referenced 82

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 11:08 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 8, 2024, 12:36 a.m.)
Issued	54 years, 2 months ago (June 1, 1971)
Published	54 years, 2 months ago (June 1, 1971)
Published Online	54 years, 2 months ago (June 1, 1971)
Published Print	54 years, 2 months ago (June 1, 1971)

Funders 0

None

BibTeX

@article{Bernhard_1971, title={IMPROVED TECHNIQUES FOR THE PREPARATION OF ULTRATHIN FROZEN SECTIONS}, volume={49}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.49.3.731}, DOI={10.1083/jcb.49.3.731}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bernhard, W. and Viron, Annie}, year={1971}, month=jun, pages={731–746} }

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