DOI: 10.1083/jcb.46.1.151. REGULATION OF INITIATION OF DNA SYNTHESIS IN CHINESE HAMSTER CELLS

REGULATION OF INITIATION OF DNA SYNTHESIS IN CHINESE HAMSTER CELLS

10.1083/jcb.46.1.151

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Suspension cultures of Chinese hamster cells (line CHO) were grown to stationary phase (approximately 8–9 x 105 cells/ml) in F-10 medium. Cells remained viable (95%) for at least 80 hr in stationary phase, and essentially all of the cells were in G1 Upon resuspension or dilution with fresh medium, the cells were induced to resume traverse of the life cycle in in synchrony, and the patterns of DNA synthesis and division were similar to those observed in cultures prepared by mitotic selection. Immediately after dilution, the rates of synthesis of RNA and protein increased threefold. This system provides a simple technique for production of large quantities of highly synchronized cells and may ultimately provide information on the biochemical mechanisms regulating cell-cycle traverse.

Bibliography

Tobey, R. A., & Ley, K. D. (1970). REGULATION OF INITIATION OF DNA SYNTHESIS IN CHINESE HAMSTER CELLS. The Journal of Cell Biology, 46(1), 151â157.

Authors 2

R. A. Tobey (first)
K. D. Ley (additional)

References 0 Referenced 118

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 10:48 p.m.)
Indexed	1 year ago (July 31, 2024, 9:33 p.m.)
Issued	55 years, 1 month ago (July 1, 1970)
Published	55 years, 1 month ago (July 1, 1970)
Published Online	55 years, 1 month ago (July 1, 1970)
Published Print	55 years, 1 month ago (July 1, 1970)

Funders 0

None

BibTeX

@article{Tobey_1970, title={REGULATION OF INITIATION OF DNA SYNTHESIS IN CHINESE HAMSTER CELLS}, volume={46}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.46.1.151}, DOI={10.1083/jcb.46.1.151}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Tobey, R. A. and Ley, K. D.}, year={1970}, month=jul, pages={151–157} }

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