DOI: 10.1083/jcb.37.3.610. THE EFFECT OF PREFIXATION ON THE DIAMETER OF CHROMOSOME FIBERS ISOLATED BY THE LANGMUIR TROUGH-CRITICAL POINT METHOD

THE EFFECT OF PREFIXATION ON THE DIAMETER OF CHROMOSOME FIBERS ISOLATED BY THE LANGMUIR TROUGH-CRITICAL POINT METHOD

10.1083/jcb.37.3.610

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The effect of prefixation on the diameter of chromosome fibers isolated by the Langmuir trough-critical point method has been investigated in several species of plants and animals. In barley, fibers isolated from endosperm without prefixation have an average diameter of between 240 and 250 A, and are similar in dimensions and structure to the chromosome fibers isolated from animals by this method. Chromosome fibers from other tissues of the same plant are smaller in diameter when isolated without prefixation, approximating 200 A. After prefixation in 2% buffered formalin, isolated fibers from the three barley tissues studied are reduced in diameter, to approximately 120–130 A for endosperm and leaflet and to 140 A for root tip. Chromosome fibers isolated from newt erythrocytes also show a significantly reduced diameter after formalin prefixation, to approximately 120 A.

Bibliography

Wolfe, S. L. (1968). THE EFFECT OF PREFIXATION ON THE DIAMETER OF CHROMOSOME FIBERS ISOLATED BY THE LANGMUIR TROUGH-CRITICAL POINT METHOD. The Journal of Cell Biology, 37(3), 610â620.

Authors 1

Stephen L. Wolfe (first)

References 0 Referenced 18

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:17 a.m.)
Indexed	2 years, 1 month ago (July 22, 2023, 10:40 a.m.)
Issued	57 years, 3 months ago (June 1, 1968)
Published	57 years, 3 months ago (June 1, 1968)
Published Online	21 years, 6 months ago (Feb. 23, 2004)
Published Print	57 years, 3 months ago (June 1, 1968)

Funders 0

None

BibTeX

@article{Wolfe_1968, title={THE EFFECT OF PREFIXATION ON THE DIAMETER OF CHROMOSOME FIBERS ISOLATED BY THE LANGMUIR TROUGH-CRITICAL POINT METHOD}, volume={37}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.37.3.610}, DOI={10.1083/jcb.37.3.610}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wolfe, Stephen L.}, year={1968}, month=jun, pages={610–620} }

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