DOI: 10.1083/jcb.35.3.675. THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER

THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER

10.1083/jcb.35.3.675

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light and electron microscopy, approximately 75% of the isolated cells retain their structural integrity. The cell suspensions are capable of maintaining endogenous respiration in the presence of 1% albumin for periods of time up to 8 hr. These cell preparations consist almost entirely of parenchymal cells and offer a unique tissue preparation for the study of hepatic metabolism.

Bibliography

Howard, R. B., Christensen, A. K., Gibbs, F. A., & Pesch, L. A. (1967). THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER. The Journal of Cell Biology, 35(3), 675â684.

Authors 4

Roger B. Howard (first)
A. Kent Christensen (additional)
Frederic A. Gibbs (additional)
Leroy A. Pesch (additional)

References 0 Referenced 164

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 9:29 p.m.)
Indexed	1 month, 3 weeks ago (July 6, 2025, 1:26 p.m.)
Issued	57 years, 8 months ago (Dec. 1, 1967)
Published	57 years, 8 months ago (Dec. 1, 1967)
Published Online	57 years, 8 months ago (Dec. 1, 1967)
Published Print	57 years, 8 months ago (Dec. 1, 1967)

Funders 0

None

BibTeX

@article{Howard_1967, title={THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER}, volume={35}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.35.3.675}, DOI={10.1083/jcb.35.3.675}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Howard, Roger B. and Christensen, A. Kent and Gibbs, Frederic A. and Pesch, Leroy A.}, year={1967}, month=dec, pages={675–684} }

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