DOI: 10.1083/jcb.34.3.773. ULTRATHIN FROZEN SECTIONS

10.1083/jcb.34.3.773

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Endogenous enzyme activity can be readily and routinely demonstrated in ultrathin, frozen sections for electron microscopy. The procedure employed to obtain the best structural preservation as well as enzyme activity in thin sections involved fixation in glutaraldehyde, embedding in thiolated gelatin or pure gelatin, partial dehydration in glycerol, and sectioning in a cryostat at -35°C with a slightly modified Porter-Blum microtome on which the tissue is maintained at -70°C and the knife at -23°C. Kidney cortex was used as test tissue, but a few other organs were occasionally used. Thin sections were floated on the surface of several incubation media routinely employed for enzyme cytochemistry. Positive, specific reactions were obtained for alkaline phosphatase in kidney brush border, for adenosine triphosphatase in brush border and in basal membranes of distal tubules, for acid phosphatase and esterase in lysosomes, and for NADH diaphorase in mitochondria. Mitochondrial ATPase was sporadically evident only in the distal tubule of the kidney. Localizations of enzyme activity reported by other technical approaches were confirmed and in some cases somewhat improved.

Bibliography

Leduc, E. H., Bernhard, W., Holt, S. J., & Tranzer, J. P. (1967). ULTRATHIN FROZEN SECTIONS. The Journal of Cell Biology, 34(3), 773â786.

Authors 4

Elizabeth H. Leduc (first)
W. Bernhard (additional)
S. J. Holt (additional)
J. P. Tranzer (additional)

References 0 Referenced 37

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years ago (July 23, 2023, 9:30 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 7, 2024, 11:19 a.m.)
Issued	57 years, 11 months ago (Sept. 1, 1967)
Published	57 years, 11 months ago (Sept. 1, 1967)
Published Online	57 years, 11 months ago (Sept. 1, 1967)
Published Print	57 years, 11 months ago (Sept. 1, 1967)

Funders 0

None

BibTeX

@article{Leduc_1967, title={ULTRATHIN FROZEN SECTIONS}, volume={34}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.34.3.773}, DOI={10.1083/jcb.34.3.773}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Leduc, Elizabeth H. and Bernhard, W. and Holt, S. J. and Tranzer, J. P.}, year={1967}, month=sep, pages={773–786} }

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