DOI: 10.1083/jcb.34.1.47. ELECTRON MICROSCOPY OF MITOSIS IN AMEBAE

ELECTRON MICROSCOPY OF MITOSIS IN AMEBAE

10.1083/jcb.34.1.47

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The mitotic apparatus (MA) of the giant ameba, Chaos carolinensis, has characteristic sequences of microtubule arrays and deployment of nuclear envelope fragments. If mitotic organisms are subjected to 2°C for 5 min, the MA microtubules are completely degraded, and the envelope fragments are released from the chromosomes which remain condensed but lose their metaphase-plate orientation. On warming, microtubules reform but show partial loss of their parallel alignment; displacement of the envelope fragments persists or is increased by microtubule reformation. This study demonstrates that cooling causes destruction of microtubules and intermicrotubular cross-bonds and further shows that such controlled dissolution and reformation can provide an in vivo test sequence for studies on the effects of inhibitor-compounds on microtubule subunit aggregation. Urea, at the comparatively low concentration of 0.8 M, inhibited reformation following cooling and rewarming but was ineffective in altering microtubules that had formed before treatment.

Bibliography

Roth, L. E. (1967). ELECTRON MICROSCOPY OF MITOSIS IN AMEBAE. The Journal of Cell Biology, 34(1), 47â59.

Authors 1

L. E. Roth (first)

References 0 Referenced 79

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 9:30 p.m.)
Indexed	1 year, 2 months ago (July 2, 2024, 5:46 a.m.)
Issued	58 years, 2 months ago (July 1, 1967)
Published	58 years, 2 months ago (July 1, 1967)
Published Online	58 years, 2 months ago (July 1, 1967)
Published Print	58 years, 2 months ago (July 1, 1967)

Funders 0

None

BibTeX

@article{Roth_1967, title={ELECTRON MICROSCOPY OF MITOSIS IN AMEBAE}, volume={34}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.34.1.47}, DOI={10.1083/jcb.34.1.47}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Roth, L. E.}, year={1967}, month=jul, pages={47–59} }

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