DOI: 10.1083/jcb.29.1.11. RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS

RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS

10.1083/jcb.29.1.11

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H3-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G1 and G2 cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.

Bibliography

Rasmussen, R. E., & Painter, R. B. (1966). RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS. The Journal of Cell Biology, 29(1), 11â19.

Authors 2

R. E. Rasmussen (first)
R. B. Painter (additional)

References 0 Referenced 215

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 2:21 p.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 6:10 a.m.)
Issued	59 years, 5 months ago (April 1, 1966)
Published	59 years, 5 months ago (April 1, 1966)
Published Online	59 years, 5 months ago (April 1, 1966)
Published Print	59 years, 5 months ago (April 1, 1966)

Funders 0

None

BibTeX

@article{Rasmussen_1966, title={RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS}, volume={29}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.29.1.11}, DOI={10.1083/jcb.29.1.11}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Rasmussen, R. E. and Painter, R. B.}, year={1966}, month=apr, pages={11–19} }

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