DOI: 10.1083/jcb.25.2.41. STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS

STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS

10.1083/jcb.25.2.41

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

DNA-protein coacervates containing colloidal gold particles were readily phagocytized by strain L fibroblasts. During the subsequent digestion process, the gold particles served as markers which permitted the demonstration of the evolution of digestive vacuoles to multivesicular bodies and finally to dense bodies. Acid phosphatase and esterolytic activity was present in these structures. The hydrolytic enzymes were apparently brought to the phagocytotic vacuoles in small vesicles originating in the Golgi region. These vesicles entered the vacuoles prior to the digestion of the coacervates and the appearance of positive cytochemical reactions. The cytoplasmic dense bodies frequently merged with the phagocytotic vacuoles. This was demonstrated by prelabeling the dense bodies with colloidal iron prior to phagocytosis of the coacervates. In addition, evidence is presented for the interrelationship of the phagocytotic and autophagic pathways.

Bibliography

Gordon, G. B., Miller, L. R., & Bensch, K. G. (1965). STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS. The Journal of Cell Biology, 25(2), 41â55.

Authors 3

Gerald B. Gordon (first)
Leonard R. Miller (additional)
Klaus G. Bensch (additional)

References 0 Referenced 169

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 6:01 p.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 1:19 p.m.)
Indexed	3 days ago (Sept. 4, 2025, 10:24 a.m.)
Issued	60 years, 4 months ago (May 1, 1965)
Published	60 years, 4 months ago (May 1, 1965)
Published Online	60 years, 4 months ago (May 1, 1965)
Published Print	60 years, 4 months ago (May 1, 1965)

Funders 0

None

BibTeX

@article{Gordon_1965, title={STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS}, volume={25}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.25.2.41}, DOI={10.1083/jcb.25.2.41}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Gordon, Gerald B. and Miller, Leonard R. and Bensch, Klaus G.}, year={1965}, month=may, pages={41–55} }

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