DOI: 10.1083/jcb.201612194. The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components

The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components

10.1083/jcb.201612194

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Kinetochores (KTs) are large multiprotein complexes that constitute the interface between centromeric chromatin and the mitotic spindle during chromosome segregation. In spite of their essential role, little is known about how centromeres and KTs are assembled and how their precise stoichiometry is regulated. In this study, we show that the nuclear pore basket component Alm1 is required to maintain both the proteasome and its anchor, Cut8, at the nuclear envelope, which in turn regulates proteostasis of certain inner KT components. Consistently, alm1-deleted cells show increased levels of KT proteins, including CENP-CCnp3, spindle assembly checkpoint activation, and chromosome segregation defects. Our data demonstrate a novel function of the nucleoporin Alm1 in proteasome localization required for KT homeostasis.

Bibliography

Salas-Pino, S., Gallardo, P., Barrales, R. R., Braun, S., & Daga, R. R. (2017). The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components. Journal of Cell Biology, 216(11), 3591â3608.

Authors 5

Silvia Salas-Pino (first)
Paola Gallardo (additional)
Ramón R. Barrales (additional)
Sigurd Braun (additional)
Rafael R. Daga (additional)

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Dates

Type	When
Created	7 years, 10 months ago (Oct. 3, 2017, 10:10 a.m.)
Deposited	2 years ago (July 23, 2023, 10:09 a.m.)
Indexed	6 months ago (Feb. 21, 2025, 4:04 a.m.)
Issued	7 years, 10 months ago (Oct. 3, 2017)
Published	7 years, 10 months ago (Oct. 3, 2017)
Published Online	7 years, 10 months ago (Oct. 3, 2017)
Published Print	7 years, 9 months ago (Nov. 6, 2017)

Funders 2

Ministerio de Economía y Competitividad 10.13039/501100003329
Region: Europe
gov (National government)
Labels3
1. Ministry of Economy and Competitiveness
2. MINECO
3. MEC
Awards1
1. BFU2015-70604-P
Universidad Pablo de Olavide
Awards1
1. Beca Puente PP1-1402

BibTeX

@article{Salas_Pino_2017, title={The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components}, volume={216}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.201612194}, DOI={10.1083/jcb.201612194}, number={11}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Salas-Pino, Silvia and Gallardo, Paola and Barrales, Ramón R. and Braun, Sigurd and Daga, Rafael R.}, year={2017}, month=oct, pages={3591–3608} }

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