DOI: 10.1083/jcb.201311104. A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells

A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells

10.1083/jcb.201311104

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

How adherent and contractile systems coordinate to promote cell shape changes is unclear. Here, we define a counterbalanced adhesion/contraction model for cell shape control. Live-cell microscopy data showed a crucial role for a contractile meshwork at the top of the cell, which is composed of actin arcs and myosin IIA filaments. The contractile actin meshwork is organized like muscle sarcomeres, with repeating myosin II filaments separated by the actin bundling protein α-actinin, and is mechanically coupled to noncontractile dorsal actin fibers that run from top to bottom in the cell. When the meshwork contracts, it pulls the dorsal fibers away from the substrate. This pulling force is counterbalanced by the dorsal fibers’ attachment to focal adhesions, causing the fibers to bend downward and flattening the cell. This model is likely to be relevant for understanding how cells configure themselves to complex surfaces, protrude into tight spaces, and generate three-dimensional forces on the growth substrate under both healthy and diseased conditions.

Bibliography

Burnette, D. T., Shao, L., Ott, C., Pasapera, A. M., Fischer, R. S., Baird, M. A., Der Loughian, C., Delanoe-Ayari, H., Paszek, M. J., Davidson, M. W., Betzig, E., & Lippincott-Schwartz, J. (2014). A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. Journal of Cell Biology, 205(1), 83â96.

Authors 12

Dylan T. Burnette (first)
Lin Shao (additional)
Carolyn Ott (additional)
Ana M. Pasapera (additional)
Robert S. Fischer (additional)
Michelle A. Baird (additional)
Christelle Der Loughian (additional)
Helene Delanoe-Ayari (additional)
Matthew J. Paszek (additional)
Michael W. Davidson (additional)
Eric Betzig (additional)
Jennifer Lippincott-Schwartz (additional)

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Dates

Type	When
Created	11 years, 4 months ago (April 8, 2014, 1:54 a.m.)
Deposited	2 years, 1 month ago (July 23, 2023, 2:35 a.m.)
Indexed	1 week, 2 days ago (Aug. 21, 2025, 1:02 p.m.)
Issued	11 years, 4 months ago (April 7, 2014)
Published	11 years, 4 months ago (April 7, 2014)
Published Online	11 years, 4 months ago (April 7, 2014)
Published Print	11 years, 4 months ago (April 14, 2014)

Funders 0

None

BibTeX

@article{Burnette_2014, title={A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells}, volume={205}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201311104}, DOI={10.1083/jcb.201311104}, number={1}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Burnette, Dylan T. and Shao, Lin and Ott, Carolyn and Pasapera, Ana M. and Fischer, Robert S. and Baird, Michelle A. and Der Loughian, Christelle and Delanoe-Ayari, Helene and Paszek, Matthew J. and Davidson, Michael W. and Betzig, Eric and Lippincott-Schwartz, Jennifer}, year={2014}, month=apr, pages={83–96} }

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