DOI: 10.1083/jcb.201201074. Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation

Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation

10.1083/jcb.201201074

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Hsp100 and Hsp70 chaperones in bacteria, yeast, and plants cooperate to reactivate aggregated proteins. Disaggregation relies on Hsp70 function and on ATP-dependent threading of aggregated polypeptides through the pore of the Hsp100 AAA+ hexamer. In yeast, both chaperones also promote propagation of prions by fibril fragmentation, but their functional interplay is controversial. Here, we demonstrate that Hsp70 chaperones were essential for species-specific targeting of their Hsp100 partner chaperones ClpB and Hsp104, respectively, to heat-induced protein aggregates in vivo. Hsp70 inactivation in yeast also abrogated Hsp104 targeting to almost all prions tested and reduced fibril mobility, which indicates that fibril fragmentation by Hsp104 requires Hsp70. The Sup35 prion was unique in allowing Hsp70-independent association of Hsp104 via its N-terminal domain, which, however, was nonproductive. Hsp104 overproduction even outcompeted Hsp70 for Sup35 prion binding, which explains why this condition prevented Sup35 fragmentation and caused prion curing. Our findings indicate a conserved mechanism of Hsp70–Hsp100 cooperation at the surface of protein aggregates and prion fibrils.

Bibliography

Winkler, J., Tyedmers, J., Bukau, B., & Mogk, A. (2012). Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation. Journal of Cell Biology, 198(3), 387â404.

Authors 4

Juliane Winkler (first)
Jens Tyedmers (additional)
Bernd Bukau (additional)
Axel Mogk (additional)

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Dates

Type	When
Created	13 years ago (Aug. 6, 2012, 12:23 p.m.)
Deposited	1 year, 3 months ago (April 28, 2024, 2:19 a.m.)
Indexed	3 weeks, 6 days ago (July 28, 2025, 5:50 p.m.)
Issued	13 years ago (Aug. 6, 2012)
Published	13 years ago (Aug. 6, 2012)
Published Online	13 years ago (Aug. 6, 2012)
Published Print	13 years ago (Aug. 6, 2012)

Funders 0

None

BibTeX

@article{Winkler_2012, title={Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation}, volume={198}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201201074}, DOI={10.1083/jcb.201201074}, number={3}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Winkler, Juliane and Tyedmers, Jens and Bukau, Bernd and Mogk, Axel}, year={2012}, month=aug, pages={387–404} }

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