DOI: 10.1083/jcb.201110047. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes

Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes

10.1083/jcb.201110047

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23CORE, revealing a binding chain of TIM23CORE-Mgr2/Tim21–respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.

Bibliography

Gebert, M., Schrempp, S. G., Mehnert, C. S., HeiÃwolf, A. K., Oeljeklaus, S., Ieva, R., Bohnert, M., von der Malsburg, K., Wiese, S., Kleinschroth, T., Hunte, C., Meyer, H. E., Haferkamp, I., Guiard, B., Warscheid, B., Pfanner, N., & van der Laan, M. (2012). Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. Journal of Cell Biology, 197(5), 595â604.

Authors 17

Michael Gebert (first)
Sandra G. Schrempp (additional)
Carola S. Mehnert (additional)
Anna K. Heißwolf (additional)
Silke Oeljeklaus (additional)
Raffaele Ieva (additional)
Maria Bohnert (additional)
Karina von der Malsburg (additional)
Sebastian Wiese (additional)
Thomas Kleinschroth (additional)
Carola Hunte (additional)
Helmut E. Meyer (additional)
Ilka Haferkamp (additional)
Bernard Guiard (additional)
Bettina Warscheid (additional)
Nikolaus Pfanner (additional)
Martin van der Laan (additional)

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Dates

Type	When
Created	13 years, 3 months ago (May 21, 2012, 10:50 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:27 p.m.)
Indexed	1 day, 13 hours ago (Aug. 29, 2025, 6:15 a.m.)
Issued	13 years, 3 months ago (May 21, 2012)
Published	13 years, 3 months ago (May 21, 2012)
Published Online	13 years, 3 months ago (May 21, 2012)
Published Print	13 years, 3 months ago (May 28, 2012)

Funders 0

None

BibTeX

@article{Gebert_2012, title={Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes}, volume={197}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201110047}, DOI={10.1083/jcb.201110047}, number={5}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Gebert, Michael and Schrempp, Sandra G. and Mehnert, Carola S. and Heißwolf, Anna K. and Oeljeklaus, Silke and Ieva, Raffaele and Bohnert, Maria and von der Malsburg, Karina and Wiese, Sebastian and Kleinschroth, Thomas and Hunte, Carola and Meyer, Helmut E. and Haferkamp, Ilka and Guiard, Bernard and Warscheid, Bettina and Pfanner, Nikolaus and van der Laan, Martin}, year={2012}, month=may, pages={595–604} }

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  "abstract": "<jats:p>Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23CORE, revealing a binding chain of TIM23CORE-Mgr2/Tim21\u2013respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.</jats:p>",
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          "name": "Fakult\u00e4t f\u00fcr Biologie 1 , 2 , 3 , and 4"
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          "name": "Centre for Biological Signalling Studies 1 , 2 , 3 , and 4"
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        {
          "name": "Institut f\u00fcr Biologie II, Funktionelle Proteomik, Fakult\u00e4t f\u00fcr Biologie, Universit\u00e4t Freiburg, 79104 Freiburg, Germany 1 , 2 , 3 , and 4"
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