DOI: 10.1083/jcb.201103132. Cbp3–Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome <i>b</i> synthesis and assembly

Cbp3–Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly

10.1083/jcb.201103132

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Mitochondria contain their own genetic system to express a small number of hydrophobic polypeptides, including cytochrome b, an essential subunit of the bc1 complex of the respiratory chain. In this paper, we show in yeast that Cbp3, a bc1 complex assembly factor, and Cbp6, a regulator of cytochrome b translation, form a complex that associates with the polypeptide tunnel exit of mitochondrial ribosomes and that exhibits two important functions in the biogenesis of cytochrome b. On the one hand, the interaction of Cbp3 and Cbp6 with mitochondrial ribosomes is necessary for efficient translation of cytochrome b transcript. On the other hand, the Cbp3–Cbp6 complex interacts directly with newly synthesized cytochrome b in an assembly intermediate that is not ribosome bound and that contains the assembly factor Cbp4. Our results suggest that synthesis of cytochrome b occurs preferentially on those ribosomes that have the Cbp3–Cbp6 complex bound to their tunnel exit, an arrangement that may ensure tight coordination of cytochrome b synthesis and assembly.

Authors 8

Steffi Gruschke (first)
Kirsten Kehrein (additional)
Katharina Römpler (additional)
Kerstin Gröne (additional)
Lars Israel (additional)
Axel Imhof (additional)
Johannes M. Herrmann (additional)
Martin Ott (additional)

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Dates

Type	When
Created	14 years, 2 months ago (June 13, 2011, 4:58 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:42 p.m.)
Indexed	1 month ago (July 25, 2025, 6:46 a.m.)
Issued	14 years, 2 months ago (June 13, 2011)
Published	14 years, 2 months ago (June 13, 2011)
Published Online	14 years, 2 months ago (June 13, 2011)
Published Print	14 years, 2 months ago (June 13, 2011)

Funders 0

None

BibTeX

@article{Gruschke_2011, title={Cbp3–Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly}, volume={193}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201103132}, DOI={10.1083/jcb.201103132}, number={6}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Gruschke, Steffi and Kehrein, Kirsten and Römpler, Katharina and Gröne, Kerstin and Israel, Lars and Imhof, Axel and Herrmann, Johannes M. and Ott, Martin}, year={2011}, month=jun, pages={1101–1114} }

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