DOI: 10.1083/jcb.201004041. Hsp70 gene association with nuclear speckles is Hsp70 promoter specific

Hsp70 gene association with nuclear speckles is Hsp70 promoter specific

10.1083/jcb.201004041

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Many mammalian genes localize near nuclear speckles, nuclear bodies enriched in ribonucleic acid–processing factors. In this paper, we dissect cis-elements required for nuclear speckle association of the heat shock protein 70 (Hsp70) locus. We show that speckle association is a general property of Hsp70 bacterial artificial chromosome transgenes, independent of the chromosome integration site, and can be recapitulated using a 2.8-kilobase HSPA1A gene fragment. Association of Hsp70 transgenes and their transcripts with nuclear speckles is transcription dependent, independent of the transcribed sequence identity, but dependent on the Hsp70 promoter sequence. Transgene speckle association does not correlate with the amount of transcript accumulation, with large transgene arrays driven by different promoters showing no speckle association, but smaller Hsp70 transgene arrays with lower transcript accumulation showing high speckle association. Moreover, despite similar levels of transcript accumulation, Hsp70 transgene speckle association is observed after heat shock but not cadmium treatment. We suggest that certain promoters may direct specific chromatin and/or transcript ribonucleoprotein modifications, leading to nuclear speckle association.

Bibliography

Hu, Y., Plutz, M., & Belmont, A. S. (2010). Hsp70 gene association with nuclear speckles is Hsp70 promoter specific. Journal of Cell Biology, 191(4), 711â719.

Authors 3

Yan Hu (first)
Matt Plutz (additional)
Andrew S. Belmont (additional)

References 27 Referenced 48

10.1016/S0091-679X(08)60986-3 / Methods Cell Biol. / Fluorescence microscopy in three dimensions by Agard (1989)
10.1016/S0076-6879(03)76027-3 / Methods Enzymol. / X-chromosome inactivation in mouse embryonic stem cells: analysis of histone modifications and transcriptional activity using immunofluorescence and FISH by Chaumeil (2003)
10.1091/mbc.10.1.211 / Mol. Biol. Cell. / Ultrastructural analysis of transcription and splicing in the cell nucleus after bromo-UTP microinjection by Cmarko (1999)
10.1038/35066075 / Nat. Rev. Genet. / Chromosome territories, nuclear architecture and gene regulation in mammalian cells by Cremer (2001)
10.1016/0014-4827(78)90373-7 / Exp. Cell Res. / Ultrastructural localization of transcription sites and of RNA distribution during the cell cycle of synchronized CHO cells by Fakan (1978)
10.1002/ar.a.20336 / Anat. Rec. A Discov. Mol. Cell. Evol. Biol. / Molecular anatomy of a speckle by Hall (2006)
{'key': '2023072300293694300_bib7', 'first-page': '153', 'article-title': 'Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena', 'volume': '2', 'author': 'Hiraoka', 'year': '1991', 'journal-title': 'Semin. Cell Biol.'} / Semin. Cell Biol. / Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena by Hiraoka (1991)
10.1111/j.1365-2443.2007.01112.x / Genes Cells. / Defective mRNA in myotonic dystrophy accumulates at the periphery of nuclear splicing speckles by Holt (2007)
10.1083/jcb.200809196 / J. Cell Biol. / Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template by Hu (2009)
10.1083/jcb.145.6.1133 / J. Cell Biol. / Intron-independent association of splicing factors with active genes by Jolly (1999)
10.1002/em.20438 / Environ. Mol. Mutagen. / Comparison of gene expression profiles in HepG2 cells exposed to arsenic, cadmium, nickel, and three model carcinogens for investigating the mechanisms of metal carcinogenesis by Kawata (2009)
10.1242/jcs.110.2.239 / J. Cell Sci. / Transcription-dependent redistribution of nuclear protein 4.1 to SC35-enriched nuclear domains by Lallena (1997)
10.1093/emboj/18.15.4308 / EMBO J. / Purification and biochemical characterization of interchromatin granule clusters by Mintz (1999)
10.1093/nar/24.15.2924 / Nucleic Acids Res. / In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin by Nguyen (1996)
{'key': '2023072300293694300_bib15', 'article-title': 'Effects of transcriptional activators on large-scale chromatin structure', 'author': 'Nye', 'year': '2003'} / Effects of transcriptional activators on large-scale chromatin structure by Nye (2003)
10.2131/jts.32.103 / J. Toxicol. Sci. / Transcriptome analysis of rat kidney cells continuously exposed to cadmium using DNA microarray by Ohba (2007)
10.1083/jcb.124.3.249 / J. Cell Biol. / Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors by O’Keefe (1994)
10.1083/jcb.200303131 / J. Cell Biol. / Clustering of multiple specific genes and gene-rich R-bands around SC-35 domains: evidence for local euchromatic neighborhoods by Shopland (2003)
10.1083/jcb.144.4.617 / J. Cell Biol. / Processing of endogenous pre-mRNAs in association with SC-35 domains is gene specific by Smith (1999)
10.1083/jcb.200706048 / J. Cell Biol. / Defining early steps in mRNA transport: mutant mRNA in myotonic dystrophy type I is blocked at entry into SC-35 domains by Smith (2007)
10.1111/j.1768-322X.1984.tb00215.x / Biol. Cell. / Immunoelectron microscopic localization of snRNPs by Spector (1983)
{'key': '2023072300293694300_bib22', 'first-page': '1035', 'article-title': 'The interchromatin granules', 'volume': '10', 'author': 'Thiry', 'year': '1995', 'journal-title': 'Histol. Histopathol.'} / Histol. Histopathol. / The interchromatin granules by Thiry (1995)
10.1083/jcb.145.7.1341 / J. Cell Biol. / Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain by Tumbar (1999)
10.1083/jcb.122.2.283 / J. Cell Biol. / Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus by Wansink (1993)
10.1093/nar/gni035 / Nucleic Acids Res. / Simple and highly efficient BAC recombineering using galK selection by Warming (2005)
{'key': '2023072300293694300_bib26', 'first-page': '3125', 'article-title': 'Maximal stress-induced transcription from the human HSP70 promoter requires interactions with the basal promoter elements independent of rotational alignment', 'volume': '10', 'author': 'Williams', 'year': '1990', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Maximal stress-induced transcription from the human HSP70 promoter requires interactions with the basal promoter elements independent of rotational alignment by Williams (1990)
10.1083/jcb.131.6.1635 / J. Cell Biol. / Nonrandom gene organization: structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains by Xing (1995)

Dates

Type	When
Created	14 years, 9 months ago (Nov. 9, 2010, 1:59 a.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 8:30 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:06 a.m.)
Issued	14 years, 9 months ago (Nov. 8, 2010)
Published	14 years, 9 months ago (Nov. 8, 2010)
Published Online	14 years, 9 months ago (Nov. 8, 2010)
Published Print	14 years, 9 months ago (Nov. 15, 2010)

Funders 0

None

BibTeX

@article{Hu_2010, title={Hsp70 gene association with nuclear speckles is Hsp70 promoter specific}, volume={191}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201004041}, DOI={10.1083/jcb.201004041}, number={4}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hu, Yan and Plutz, Matt and Belmont, Andrew S.}, year={2010}, month=nov, pages={711–719} }

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